Browsing by keyword "Ependyma"

Now showing items 1-2 of 2

    • High-speed digital imaging of ependymal cilia in the murine brain
      Lechtreck, Karl-Ferdinand; Sanderson, Michael J.; Witman, George B. (2009-01-01)
      The development and health of mammals requires proper ciliary motility. Ciliated epithelia are found in the airways, the uterus and Fallopian tubes, the efferent ducts of the testes, and the ventricular system of the brain. A technique is described for the motion analysis of ependymal cilia in the murine brain. Vibratome sections of the brain are imaged by differential interference contrast microscopy and recorded by high-speed digital imaging. Side views of individual cilia are traced to establish their bending pattern. Tracking of individual cilia recorded in top view allows determination of bend planarity and beat direction. Ciliary beat frequency is determined from line scans of image sequences. The capacity of the epithelium to move fluid and objects is revealed by analyzing the velocity of polystyrene beads added to brain sections. The technique is useful for detailed assessment of how various conditions or mutations affect the fidelity of ciliary motility at the ependyma. The methods are also applicable to other ciliated epithelia, for example, in airways.

    • Mutations in Hydin impair ciliary motility in mice
      Lechtreck, Karl-Ferdinand; Delmotte, Philippe; Robinson, Michael L.; Sanderson, Michael J.; Witman, George B. (2008-02-06)
      Chlamydomonas reinhardtii hydin is a central pair protein required for flagellar motility, and mice with Hydin defects develop lethal hydrocephalus. To determine if defects in Hydin cause hydrocephalus through a mechanism involving cilia, we compared the morphology, ultrastructure, and activity of cilia in wild-type and hydin mutant mice strains. The length and density of cilia in the brains of mutant animals is normal. The ciliary axoneme is normal with respect to the 9 + 2 microtubules, dynein arms, and radial spokes but one of the two central microtubules lacks a specific projection. The hydin mutant cilia are unable to bend normally, ciliary beat frequency is reduced, and the cilia tend to stall. As a result, these cilia are incapable of generating fluid flow. Similar defects are observed for cilia in trachea. We conclude that hydrocephalus in hydin mutants is caused by a central pair defect impairing ciliary motility and fluid transport in the brain.