Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples
Juette, Manuel F. ; Gould, Travis J. ; Lessard, Mark D. ; Mlodzianoski, Michael J. ; Nagpure, Bhupendra S. ; Bennett, Brian Thomas ; Hess, Samuel T. ; Bewersdorf, Jorg
Juette, Manuel F.
Gould, Travis J.
Lessard, Mark D.
Mlodzianoski, Michael J.
Nagpure, Bhupendra S.
Bennett, Brian Thomas
Hess, Samuel T.
Bewersdorf, Jorg
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Journal Article
Publication Date
2008-05-13
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Abstract
Imaging volumes as thick as whole cells at three-dimensional (3D) super-resolution is required to reveal unknown features of cellular organization. We report a light microscope that generates images with translationally invariant 30 x 30 x 75 nm resolution over a depth of several micrometers. This method, named biplane (BP) FPALM, combines a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) enabling 3D sub-diffraction resolution without compromising speed or sensitivity.
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Nat Methods. 2008 Jun;5(6):527-9. Epub 2008 May 11. Link to article on publisher's site
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DOI
10.1038/nmeth.1211
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PubMed ID
18469823