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Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples

Juette, Manuel F.
Gould, Travis J.
Lessard, Mark D.
Mlodzianoski, Michael J.
Nagpure, Bhupendra S.
Bennett, Brian Thomas
Hess, Samuel T.
Bewersdorf, Jorg
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Abstract

Imaging volumes as thick as whole cells at three-dimensional (3D) super-resolution is required to reveal unknown features of cellular organization. We report a light microscope that generates images with translationally invariant 30 x 30 x 75 nm resolution over a depth of several micrometers. This method, named biplane (BP) FPALM, combines a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) enabling 3D sub-diffraction resolution without compromising speed or sensitivity.

Source

Nat Methods. 2008 Jun;5(6):527-9. Epub 2008 May 11. Link to article on publisher's site

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DOI
10.1038/nmeth.1211
PubMed ID
18469823
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