Publication

Neuroprotective effect of hyperbaric oxygen therapy monitored by MR-imaging after embolic stroke in rats

Henninger, Nils
Kuppers-Tiedt, Lea
Sicard, Kenneth M.
Gunther, Albrecht
Schneider, Dietmar
Schwab, Stefan
Citations
Altmetric:
Student Authors
Kenneth Sicard
Faculty Advisor
Academic Program
MD/PhD
Document Type
Journal Article
Publication Date
2006-07-04
Keywords
Subject Area
Embargo Expiration Date
Abstract

The potential neuroprotective effects of hyperbaric oxygen (HBO) were tested in an embolic model of focal cerebral ischemia with partially spontaneous reperfusion. Rats (n = 10) were subjected to embolic middle cerebral artery occlusion (MCAO) and diffusion weighted MRI (DWI) was performed at baseline, 1, 3, and 6 h after MCAO to determine the ADC viability threshold yielding the lesion volumes that best approximated the 2,3,5-triphenyltetrazolium chloride (TTC) infarct volumes at 24 h (experiment 1). For assessment of neuroprotective effects, rats were treated with 100% oxygen at 2.5 atmospheres absolute (ATA, n = 15) or normobaric room air (n = 15) for 60 min beginning 180 min after MCAO (experiment 2). DWI-, perfusion (PWI)- and T2-weighted MRI (T2WI) started within 0.5 h after MCAO and was continued 5 h, 24 h (PWI and T2WI only), and 168 h (T2WI only). Infarct volume was calculated based on TTC-staining at 24 h (experiment 1) or 168 h (experiment 2) post-MCAO. ADC-lesion evolution was maximal between 3 and 6 h. In experiment 2, the relative regional cerebral blood volume (rCBV) of both groups showed similar incomplete spontaneous reperfusion in the ischemic core. HBO reduced infarct volume to 145.3 +/- 39.6 mm3 vs. 202.5 +/- 58.3 mm3 (control, P = 0.029). As shown by MRI and TTC, HBO treatment demonstrated significant neuroprotection at 5 h after embolic focal cerebral ischemia that lasted for 168 h.

Source
Year of Medical School at Time of Visit
Sponsors
Dates of Travel
DOI
PubMed ID
16814772
Other Identifiers
Notes

Co-author Kenneth Sicard is a doctoral student in the MD/PhD Program in the Morningside Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.

Funding and Acknowledgements
Corresponding Author
Related Resources
Related Resources
Repository Citation
Rights
Citation: Exp Neurol. 2006 Oct;201(2):316-23. Epub 2006 Jun 30. <a href="http://dx.doi.org/10.1016/j.expneurol.2006.04.011">Link to article on publisher's site</a>
Distribution License