Growth-rate-dependent transcription initiation from the dam P2 promoter
Rasmussen, Lene Juel Lobner-Olesen, Anders Marinus, Martin G.
Rasmussen, Lene Juel
Lobner-Olesen, Anders
Marinus, Martin G.
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Faculty Advisor
Academic Program
UMass Chan Affiliations
Document Type
Journal Article
Publication Date
1995-05-19
Keywords
Base Sequence
Colon
Escherichia coli
Escherichia coli Proteins
*Gene Expression Regulation, Bacterial
Humans
Methyltransferases
*Promoter Regions, Genetic
Recombinant Fusion Proteins
*Site-Specific DNA-Methyltransferase (Adenine-Specific)
Substrate Specificity
beta-Galactosidase
DNA methylation
lipoic acid ligase
recombinant DNA
gene regulation
Bacteria
Biochemistry, Biophysics, and Structural Biology
Genetic Phenomena
Pharmacology, Toxicology and Environmental Health
Colon
Escherichia coli
Escherichia coli Proteins
*Gene Expression Regulation, Bacterial
Humans
Methyltransferases
*Promoter Regions, Genetic
Recombinant Fusion Proteins
*Site-Specific DNA-Methyltransferase (Adenine-Specific)
Substrate Specificity
beta-Galactosidase
DNA methylation
lipoic acid ligase
recombinant DNA
gene regulation
Bacteria
Biochemistry, Biophysics, and Structural Biology
Genetic Phenomena
Pharmacology, Toxicology and Environmental Health
Subject Area
Embargo Expiration Date
Link to Full Text
Abstract
Transcription of the dam gene in Escherichia coli is dependent on growth rate. Using single-copy promoter::lacZYA fusions we found that of the five promoter regions which affect dam expression, only the P2 promoter shows growth-rate dependence. The determinants for growth-rate control must lie in the region -52 to +27 relative to the transcription start point.
Source
Gene. 1995 May 19;157(1-2):213-5.
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DOI
10.1016/0378-1119(94)00619-4
Permanent Link to this Item
PubMed ID
7607492