The zebrafish (Danio rerio) has emerged as a powerful model to study vertebrate development and disease. Its short generation time makes it amenable to genetic manipulation and analysis, and its small size and high fecundity make it especially well suited for large-scale forward genetic and chemical screens. Fast-developing zebrafish embryos are transparent, facilitating live imaging of a variety of developmental processes in wild-type and mutant animals. ...

This brief document provides an updated set of guidelines regarding morpholino use in zebrafish that we anticipate will be of value for experimentalists as well as journal and grant reviewers, and decision makers.