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RNA Interference in Caenorhabditis elegans

Conte, Darryl Jr.
; 
MacNeil, Lesley T.
; 
Walhout, Albertha J M
; 
Mello, Craig C.
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Authors
Conte, Darryl Jr.
MacNeil, Lesley T.
Walhout, Albertha J M
Mello, Craig C.
Student Authors
Faculty Advisor
Academic Program
UMass Chan Affiliations
Program in Molecular Medicine
 RNA Therapeutics Institute (RTI)
 Program in Systems Biology
Document Type
Journal Article
Publication Date
2015-01-05
Keywords
Genetics and Genomics
 Laboratory and Basic Science Research
 Molecular Biology
 Systems Biology
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UMass Chan Faculty and Researcher Publications
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Link to Full Text
http://dx.doi.org/10.1002/0471142727.mb2603s109
Abstract

RNAi has become an essential tool in C. elegans research. This unit describes procedures for RNAi in C. elegans by microinjecting with dsRNA, feeding with bacteria expressing dsRNA, and soaking in dsRNA solution, as well as high-throughput methods for RNAi-based screens. (c) 2015 by John Wiley and Sons, Inc.

Source

Curr Protoc Mol Biol. 2015 Jan 5;109:26.3.1-26.3.30. doi: 10.1002/0471142727.mb2603s109. Link to article on publisher's site

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DOI
10.1002/0471142727.mb2603s109
Permanent Link to this Item
https://hdl.handle.net/20.500.14038/49942
PubMed ID
25559107
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