The RNA phosphatase PIR-1 regulates endogenous small RNA pathways in C. elegans [preprint]
Chaves, Daniel A. ; Dai, Hui ; Li, Lichao ; Moresco, James J. ; Eun Oh, Myung ; Conte, Darryl Jr. ; Yates, John R. III ; Mello, Craig C. ; Gu, Weifeng
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Abstract
Eukaryotic cells regulate 5' triphosphorylated (ppp-) RNAs to promote cellular functions and prevent recognition by antiviral RNA sensors. For example, RNA capping enzymes possess triphosphatase domains that remove the γ phosphates of ppp-RNAs during RNA capping. Members of the closely related PIR1 family of RNA polyphosphatases remove both the β and γ phosphates from ppp-RNAs. Here we show that C. elegans PIR-1 dephosphorylates ppp-RNAs made by cellular RdRPs and is required for the maturation of 26G-RNAs, Dicer-dependent small RNAs that regulate thousands of genes during spermatogenesis and embryogenesis. PIR-1 also regulates the CSR-1 22G-RNA pathway and has critical functions in both somatic and germline development. Our findings suggest that PIR-1 modulates both Dicer-dependent and -independent Argonaute pathways, and provide insight into how cells and viruses use a conserved RNA phosphatase to regulate and respond to ppp-RNA species.
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bioRxiv 2020.08.03.235143; doi: https://doi.org/10.1101/2020.08.03.235143. Link to preprint on bioRxiv service.
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Now published in Molecular Cell doi: 10.1016/j.molcel.2020.12.004