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High-Efficiency Yeast Transformation

Reece-Hoyes, John S.
; 
Walhout, Albertha J M
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Authors
Reece-Hoyes, John S.
Walhout, Albertha J M
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Faculty Advisor
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UMass Chan Affiliations
Program in Molecular Medicine
 Program in Systems Biology
Document Type
Journal Article
Publication Date
2018-07-02
Keywords
Genetics and Genomics
 Laboratory and Basic Science Research
 Systems Biology
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UMass Chan Faculty and Researcher Publications
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https://doi.org/10.1101/pdb.prot094995
Abstract

High-efficiency yeast transformation is used for integrations into YM4271 (yeast one-hybrid (Y1H) DNA-bait generation), for transforming libraries of activation domain (AD)-prey clones into Y1H and yeast two-hybrid (Y2H)-bait strains, and for gap repair. The protocol takes 2 d to complete.

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Cold Spring Harb Protoc. 2018 Jul 2;2018(7):pdb.prot094995. doi: 10.1101/pdb.prot094995.Link to article on publisher's site

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DOI
10.1101/pdb.prot094995
Permanent Link to this Item
https://hdl.handle.net/20.500.14038/49869
PubMed ID
29967274
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