Design, Cloning, and In Vitro Screening of Artificial miRNAs to Silence Alpha-1 Antitrypsin
Borel, Florie Mueller, Christian
Borel, Florie
Mueller, Christian
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UMass Chan Affiliations
Document Type
Book Chapter
Publication Date
2017-07-28
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Abstract
This protocol describes the design, cloning, and in vitro screening of artificial microRNAs (miRNAs) to silence alpha-1 antitrypsin (AAT). This method would be of interest to silence AAT in a variety of in vitro or in vivo models, and prevalidated sequences against human AAT are provided. This simple 5-day protocol may more generally be used to design artificial miRNAs against any transcript.
Source
Methods Mol Biol. 2017;1639:107-114. doi: 10.1007/978-1-4939-7163-3_10. Link to article on publisher's site
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DOI
10.1007/978-1-4939-7163-3_10
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PubMed ID
28752450