Identification of a weak promoter for the dam gene of Escherichia coli
Wu, Te-hui ; Grelland, Ellen ; Boye, Erik ; Marinus, Martin G.
Wu, Te-hui
Grelland, Ellen
Boye, Erik
Marinus, Martin G.
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UMass Chan Affiliations
Document Type
Journal Article
Publication Date
1992-05-07
Keywords
Base Sequence
Cloning, Molecular
Escherichia coli
Escherichia coli Proteins
Lac Operon
Methyltransferases
Molecular Sequence Data
*Promoter Regions, Genetic
*Site-Specific DNA-Methyltransferase (Adenine-Specific)
Transcription, Genetic
beta-Galactosidase
Biochemistry, Biophysics, and Structural Biology
Pharmacology, Toxicology and Environmental Health
Cloning, Molecular
Escherichia coli
Escherichia coli Proteins
Lac Operon
Methyltransferases
Molecular Sequence Data
*Promoter Regions, Genetic
*Site-Specific DNA-Methyltransferase (Adenine-Specific)
Transcription, Genetic
beta-Galactosidase
Biochemistry, Biophysics, and Structural Biology
Pharmacology, Toxicology and Environmental Health
Subject Area
Embargo Expiration Date
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Abstract
We have used a combination of techniques to identify a weak promoter located about 70 nucleotides before the start site of translation of the Escherichia coli dam gene which encodes a DNA methyltransferase. The promoter activity was identified by the use of lacZ fusions to fragments containing different lengths of upstream DNA. In vitro run-off transcription and primer extension determinations revealed transcription initiation sites at either 69 or 73 nucleotides prior to the ATG of the dam coding sequence. No ribosome binding sequence was present close to the ATG codon suggesting that the transcript may be inefficiently translated.
Source
Biochim Biophys Acta. 1992 May 7;1131(1):47-52.
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PubMed ID
1581360