Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia
Souders, Colby A. Maynard, Sharon E. Yan, Jing Wang, Yan Boatright, Naomi Sedan, Jessica Balyozian, David Cheslock, Peter S. Molrine, Deborah C. Moore Simas, Tiffany A
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Abstract
Angiogenic biomarkers, including soluble fms-like tyrosine kinase 1 (sFlt1), are thought to be predictors of preeclampsia onset; however, improvement is needed before a widespread diagnostic test can be utilized. Here we describe the development and use of diagnostic monoclonal antibodies specific to the two main splice variants of sFlt1, sFlt1-1 and sFlt1-14. These antibodies were selected for their sensitivity and specificity to their respective sFlt1 isoform in a capture ELISA format. Data from this pilot study suggest that sFlt1-1 may be more predictive of preeclampsia than total sFlt1. It may be possible to improve current diagnostic platforms if more specific antibodies are utilized.
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Int J Mol Sci. 2015 Jun 2;16(6):12436-53. doi: 10.3390/ijms160612436. Link to article on publisher's site
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This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.