Dormancy-to-death transition in yeast spores occurs due to gradual loss of gene-expressing ability

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dc.contributor.authorMaire, Theo
dc.contributor.authorAllertz, Tim
dc.contributor.authorBetjes, Max A.
dc.contributor.authorYouk, Hyun
dc.contributor.departmentProgram in Systems Biology
dc.contributor.departmentProgram in Molecular Medicine
dc.date2022-08-11T08:09:58.000
dc.date.accessioned2022-08-23T16:50:33Z
dc.date.available2022-08-23T16:50:33Z
dc.date.issued2020-11-18
dc.date.submitted2021-01-07
dc.description.abstractDormancy is colloquially considered as extending lifespan by being still. Starved yeasts form dormant spores that wake-up (germinate) when nutrients reappear but cannot germinate (die) after some time. What sets their lifespans and how they age are open questions because what processes occur-and by how much-within each dormant spore remains unclear. With single-cell-level measurements, we discovered how dormant yeast spores age and die: spores have a quantifiable gene-expressing ability during dormancy that decreases over days to months until it vanishes, causing death. Specifically, each spore has a different probability of germinating that decreases because its ability to-without nutrients-express genes decreases, as revealed by a synthetic circuit that forces GFP expression during dormancy. Decreasing amounts of molecules required for gene expression-including RNA polymerases-decreases gene-expressing ability which then decreases chances of germinating. Spores gradually lose these molecules because they are produced too slowly compared with their degradations, causing gene-expressing ability to eventually vanish and, thus, death. Our work provides a systems-level view of dormancy-to-death transition.
dc.identifier.citation<p>Maire T, Allertz T, Betjes MA, Youk H. Dormancy-to-death transition in yeast spores occurs due to gradual loss of gene-expressing ability. Mol Syst Biol. 2020 Nov;16(11):e9245. doi: 10.15252/msb.20199245. PMID: 33206464; PMCID: PMC7673291. <a href="https://doi.org/10.15252/msb.20199245">Link to article on publisher's site</a></p>
dc.identifier.contextkey20969183
dc.identifier.doi10.15252/msb.20199245
dc.identifier.issn1744-4292 (Linking)
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/4472
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5502&amp;context=oapubs&amp;unstamped=1
dc.identifier.pmid33206464
dc.identifier.submissionpathoapubs/4472
dc.identifier.urihttps://hdl.handle.net/20.500.14038/41682
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=33206464&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright 2020 The Authors. Published under the terms of the CC BY 4.0 license.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.source.issue11
dc.source.journaltitleMolecular systems biology
dc.source.pagese9245
dc.source.volume16
dc.subjectageing
dc.subjectdormancy
dc.subjectgene expression
dc.subjectgermination
dc.subjectyeast spores
dc.subjectCell Biology
dc.subjectCellular and Molecular Physiology
dc.subjectFungi
dc.subjectMolecular Biology
dc.subjectSystems Biology
dc.titleDormancy-to-death transition in yeast spores occurs due to gradual loss of gene-expressing ability
dc.typeJournal Article
dspace.entity.typePublication
html.description.abstract<p>Dormancy is colloquially considered as extending lifespan by being still. Starved yeasts form dormant spores that wake-up (germinate) when nutrients reappear but cannot germinate (die) after some time. What sets their lifespans and how they age are open questions because what processes occur-and by how much-within each dormant spore remains unclear. With single-cell-level measurements, we discovered how dormant yeast spores age and die: spores have a quantifiable gene-expressing ability during dormancy that decreases over days to months until it vanishes, causing death. Specifically, each spore has a different probability of germinating that decreases because its ability to-without nutrients-express genes decreases, as revealed by a synthetic circuit that forces GFP expression during dormancy. Decreasing amounts of molecules required for gene expression-including RNA polymerases-decreases gene-expressing ability which then decreases chances of germinating. Spores gradually lose these molecules because they are produced too slowly compared with their degradations, causing gene-expressing ability to eventually vanish and, thus, death. Our work provides a systems-level view of dormancy-to-death transition.</p>
refterms.dateFOA2022-08-23T16:50:34Z
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