Controlling gene expression in living cells through small molecule-RNA interactions
Werstuck, Geoffrey ; Green, Michael R.
Werstuck, Geoffrey
Green, Michael R.
Citations
Altmetric:
Authors
Student Authors
Faculty Advisor
Academic Program
UMass Chan Affiliations
Document Type
Journal Article
Publication Date
1998-10-09
Keywords
Animals
Anti-Bacterial Agents
Base Sequence
Benzimidazoles
Bisbenzimide
CHO Cells
Cricetinae
Drug Resistance, Microbial
Escherichia coli
*Gene Expression Regulation
Kanamycin
Ligands
Molecular Sequence Data
Protein Biosynthesis
RNA
RNA, Messenger
Tobramycin
Transfection
Life Sciences
Medicine and Health Sciences
Anti-Bacterial Agents
Base Sequence
Benzimidazoles
Bisbenzimide
CHO Cells
Cricetinae
Drug Resistance, Microbial
Escherichia coli
*Gene Expression Regulation
Kanamycin
Ligands
Molecular Sequence Data
Protein Biosynthesis
RNA
RNA, Messenger
Tobramycin
Transfection
Life Sciences
Medicine and Health Sciences
Subject Area
Embargo Expiration Date
Link to Full Text
Abstract
Short RNA aptamers that specifically bind to a wide variety of ligands in vitro can be isolated from randomized pools of RNA. Here it is shown that small molecule aptamers also bound their ligand in vivo, enabling development of a method for controlling gene expression in living cells. Insertion of a small molecule aptamer into the 5' untranslated region of a messenger RNA allowed its translation to be repressible by ligand addition in vitro as well as in mammalian cells. The ability of small molecules to control expression of specific genes could facilitate studies in many areas of biology and medicine.
Source
Science. 1998 Oct 9;282(5387):296-8.
Year of Medical School at Time of Visit
Sponsors
Dates of Travel
DOI
10.1126/science.282.5387.296
Permanent Link to this Item
PubMed ID
9765156