The NIT1 promoter allows inducible and reversible silencing of centrin in Chlamydomonas reinhardtii

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dc.contributor.authorKoblenz, Bettina
dc.contributor.authorLechtreck, Karl-Ferdinand
dc.contributor.departmentDepartment of Cell Biology
dc.date2022-08-11T08:10:02.000
dc.date.accessioned2022-08-23T16:53:01Z
dc.date.available2022-08-23T16:53:01Z
dc.date.issued2005-11-10
dc.date.submitted2008-07-09
dc.description.abstractAn inverted repeat corresponding to parts of the centrin gene of Chlamydomonas reinhardtii was placed downstream of the NIT1 promoter, which is induced by ammonium starvation. After induction, transformants developed centrin deficiency as assayed by immunofluorescence, Western blotting, and Northern blotting. The effect was reversible, demonstrating that the NIT1 promoter allowed controlled RNA interference in Chlamydomonas reinhardtii.
dc.identifier.citationEukaryot Cell. 2005 Nov;4(11):1959-62. <a href="http://dx.doi.org/10.1128/EC.4.11.1959-1962.2005">Link to article on publisher's site</a>
dc.identifier.contextkey545032
dc.identifier.doi10.1128/EC.4.11.1959-1962.2005
dc.identifier.issn1535-9778 (Print)
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/550
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1549&amp;context=oapubs&amp;unstamped=1
dc.identifier.pmid16278463
dc.identifier.submissionpathoapubs/550
dc.identifier.urihttps://hdl.handle.net/20.500.14038/42180
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=16278463&dopt=Abstract">Link to Article in PubMed</a>
dc.source.issue11
dc.source.journaltitleEukaryotic cell
dc.source.pages1959-62
dc.source.volume4
dc.subjectAminohydrolases
dc.subjectAnimals
dc.subjectChlamydomonas reinhardtii
dc.subject*Gene Silencing
dc.subject*Promoter Regions (Genetics)
dc.subjectRNA Interference
dc.subjectTrimethoprim-Sulfamethoxazole Combination
dc.subjectCell Biology
dc.subjectGenetics and Genomics
dc.titleThe NIT1 promoter allows inducible and reversible silencing of centrin in Chlamydomonas reinhardtii
dc.typeJournal Article
dspace.entity.typePublication
html.description.abstract<p>An inverted repeat corresponding to parts of the centrin gene of Chlamydomonas reinhardtii was placed downstream of the NIT1 promoter, which is induced by ammonium starvation. After induction, transformants developed centrin deficiency as assayed by immunofluorescence, Western blotting, and Northern blotting. The effect was reversible, demonstrating that the NIT1 promoter allowed controlled RNA interference in Chlamydomonas reinhardtii.</p>
refterms.dateFOA2022-08-23T16:53:01Z
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