Cytocompatible poly(ethylene glycol)-co-polycarbonate hydrogels cross-linked by copper-free, strain-promoted click chemistry
Xu, Jianwen ; Filion, Tera M. ; Prifti, Fioleda ; Song, Jie
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UMass Chan Affiliations
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Keywords
Animals
Azides
Biocompatible Materials
Bone Marrow Cells
Cell Survival
*Click Chemistry
Cyclization
Heterocyclic Compounds, 3-Ring
Hydrogels
Male
Molecular Structure
Polyethylene Glycols
Rats
Biochemistry, Biophysics, and Structural Biology
Cell and Developmental Biology
Medicine and Health Sciences
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Abstract
Strategies to encapsulate cells in cytocompatible three-dimensional hydrogels with tunable mechanical properties and degradability without harmful gelling conditions are highly desired for regenerative medicine applications. Here we reported a method for preparing poly(ethylene glycol)-co-polycarbonate hydrogels through copper-free, strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry. Hydrogels with varying mechanical properties were formed by "clicking" azido-functionalized poly(ethylene glycol)-co-polycarbonate macromers with dibenzocyclooctyne-functionalized poly(ethylene glycol) under physiological conditions within minutes. Bone marrow stromal cells encapsulated in these gels exhibited higher cellular viability than those encapsulated in photo-cross-linked poly(ethylene glycol) dimethacrylate. The precise control over the macromer compositions, cytocompatible SPAAC cross-linking, and the degradability of the polycarbonate segments make these hydrogels promising candidates for scaffold and stem cell assisted tissue repair and regeneration.
Source
Chem Asian J. 2011 Oct 4;6(10):2730-7. doi: 10.1002/asia.201100411. Epub 2011 Aug 24. Link to article on publisher's site