A 5'-uridine Amplifies miRNA/miRNA* Asymmetry in Drosophila by Promoting RNA-induced Silencing Complex Formation

Show simplified metadata
dc.contributor.authorSeitz, Herve
dc.contributor.authorTushir, Jogender S.
dc.contributor.authorZamore, Phillip D.
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.date2022-08-11T08:09:40.000
dc.date.accessioned2022-08-23T16:39:32Z
dc.date.available2022-08-23T16:39:32Z
dc.date.issued2011-06-07
dc.date.submitted2012-03-22
dc.description.abstractBACKGROUND: MicroRNA (miRNA) are diverse in sequence and have a single known sequence bias: they tend to start with uridine (U). RESULTS: Our analyses of fly, worm and mouse miRNA sequence data reveal that the 5'-U is recognized after miRNA production. Only one of the two strands can be assembled into Argonaute protein from a single miRNA/miRNA* molecule: in fly embryo lysate, a 5'-U promotes miRNA loading while decreasing the loading of the miRNA*. CONCLUSION: We suggest that recognition of the 5'-U enhances Argonaute loading by a mechanism distinct from its contribution to weakening base pairing at the 5'-end of the prospective miRNA and, as recently proposed in Arabidopsis and in humans, that it improves miRNA precision by excluding incorrectly processed molecules bearing other 5'-nt.
dc.identifier.citationSilence. 2011 Jun 7;2:4. <a href="http://dx.doi.org/10.1186/1758-907X-2-4">Link to article on publisher's site</a>
dc.identifier.contextkey2691130
dc.identifier.doi10.1186/1758-907X-2-4
dc.identifier.issn1758-907X (Linking)
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/2289
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=3288&amp;context=oapubs&amp;unstamped=1
dc.identifier.pmid21649885
dc.identifier.pmid21649885
dc.identifier.submissionpathoapubs/2289
dc.identifier.urihttps://hdl.handle.net/20.500.14038/39493
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=21649885&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1186/1758-907X-2-4
dc.rights<p>© 2011 Seitz et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<a href="http://creativecommons.org/licenses/by/2.0">http://creativecommons.org/licenses/by/2.0</a>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p>
dc.source.journaltitleSilence
dc.source.pages4
dc.source.volume2
dc.subjectMicroRNAs
dc.subjectRNA-Induced Silencing Complex
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.subjectGenetics and Genomics
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleA 5'-uridine Amplifies miRNA/miRNA* Asymmetry in Drosophila by Promoting RNA-induced Silencing Complex Formation
dc.typeJournal Article
dspace.entity.typePublication
html.description.abstract<p>BACKGROUND: MicroRNA (miRNA) are diverse in sequence and have a single known sequence bias: they tend to start with uridine (U).</p> <p>RESULTS: Our analyses of fly, worm and mouse miRNA sequence data reveal that the 5'-U is recognized after miRNA production. Only one of the two strands can be assembled into Argonaute protein from a single miRNA/miRNA* molecule: in fly embryo lysate, a 5'-U promotes miRNA loading while decreasing the loading of the miRNA*.</p> <p>CONCLUSION: We suggest that recognition of the 5'-U enhances Argonaute loading by a mechanism distinct from its contribution to weakening base pairing at the 5'-end of the prospective miRNA and, as recently proposed in Arabidopsis and in humans, that it improves miRNA precision by excluding incorrectly processed molecules bearing other 5'-nt.</p>
refterms.dateFOA2022-08-23T16:39:32Z
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Silence_Seitz_1758_907X_2_4.pdf
Size:
321.02 KB
Format:
Adobe Portable Document Format
Download
Collections
UMass Chan Faculty and Researcher Publications