Yeast one-hybrid assays for gene-centered human gene regulatory network mapping
Reece-Hoyes, John S. Barutcu, A. Rasim McCord, Rachel Patton Jeong, Jun Seop Jiang, Lizhi Macwilliams, Andrew Yang, Xinping Salehi-Ashtiani, Kourosh Hill, David E. Blackshaw, Seth
Reece-Hoyes, John S.
Barutcu, A. Rasim
McCord, Rachel Patton
Jeong, Jun Seop
Jiang, Lizhi
Macwilliams, Andrew
Yang, Xinping
Salehi-Ashtiani, Kourosh
Hill, David E.
Blackshaw, Seth
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Ahmet Rasim Barutcu
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Journal Article
Publication Date
2011-10-30
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Abstract
Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources, including clones for 988 of 1,434 (69%) predicted human transcription factors, that can be used to detect both known and new interactions between human DNA regions and transcription factors.
Source
Nat Methods. 2011 Oct 30;8(12):1050-2. doi: 10.1038/nmeth.1764. Link to article on publisher's site
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DOI
10.1038/nmeth.1764
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PubMed ID
22037702