A DNA-guided Argonaute Protein Functions in DNA Replication in Thermus thermophilus [preprint]
Jolly, Samson M. Zhang, Han Strittmatter, Lara Hendricks, Gregory M. Dhabaria, Avantika Ueberheide, Beatrix Zamore, Phillip D.
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Abstract
Argonaute proteins use nucleic acid guides to protect organisms against transposons and viruses. In the eubacterium Thermus thermophilus, the DNA-guided Argonaute TtAgo defends against transformation by DNA plasmids. Here, we report that TtAgo also participates in DNA replication. TtAgo binds small DNA guides derived from the chromosomal region where replication terminates and associates with proteins known to act in DNA replication. T. thermophilus deploys a single type II topoisomerase, gyrase. When gyrase is inhibited, T. thermophilus relies on TtAgo to complete replication of its circular genome; loss of both gyrase and TtAgo activity produces long filaments that fail to separate into individual bacteria. We propose that the primary role of TtAgo is to help T. thermophilus disentangle the catenated circular chromosomes made by DNA replication.
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bioRxiv 869172; doi: https://doi.org/10.1101/869172. Link to preprint on bioRxiv service.
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Now published in Cell doi: 10.1016/j.cell.2020.07.036