Loading...
Thumbnail Image
Publication

MyoD targets chromatin remodeling complexes to the myogenin locus prior to forming a stable DNA-bound complex

de la Serna, Ivana L.
Ohkawa, Yasuyuki
Berkes, Charlotte A.
Bergstrom, Donald A.
Dacwag, Caroline S.
Tapscott, Stephen J.
Imbalzano, Anthony N.
Citations
Altmetric:
Student Authors
Caroline S. Dacwag
Faculty Advisor
Academic Program
UMass Chan Affiliations
Document Type
Journal Article
Publication Date
2005-05-05
Keywords
Subject Area
Embargo Expiration Date
Link to Full Text
Abstract

The activation of muscle-specific gene expression requires the coordinated action of muscle regulatory proteins and chromatin-remodeling enzymes. Microarray analysis performed in the presence or absence of a dominant-negative BRG1 ATPase demonstrated that approximately one-third of MyoD-induced genes were highly dependent on SWI/SNF enzymes. To understand the mechanism of activation, we performed chromatin immunoprecipitations analyzing the myogenin promoter. We found that H4 hyperacetylation preceded Brg1 binding in a MyoD-dependent manner but that MyoD binding occurred subsequent to H4 modification and Brg1 interaction. In the absence of functional SWI/SNF enzymes, muscle regulatory proteins did not bind to the myogenin promoter, thereby providing evidence for SWI/SNF-dependent activator binding. We observed that the homeodomain factor Pbx1, which cooperates with MyoD to stimulate myogenin expression, is constitutively bound to the myogenin promoter in a SWI/SNF-independent manner, suggesting a two-step mechanism in which MyoD initially interacts indirectly with the myogenin promoter and attracts chromatin-remodeling enzymes, which then facilitate direct binding by MyoD and other regulatory proteins.

Source

Mol Cell Biol. 2005 May;25(10):3997-4009. Link to article on publisher's site

Year of Medical School at Time of Visit
Sponsors
Dates of Travel
DOI
10.1128/MCB.25.10.3997-4009.2005
PubMed ID
15870273
Other Identifiers
Notes
Funding and Acknowledgements
Corresponding Author
Related Resources
Related Resources
Repository Citation
Rights
Distribution License