Construction and application of site-specific artificial nucleases for targeted gene editing
Kok, Fatma O. Gupta, Ankit Lawson, Nathan D. Wolfe, Scot A.
Kok, Fatma O.
Gupta, Ankit
Lawson, Nathan D.
Wolfe, Scot A.
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Ankit Gupta
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UMass Chan Affiliations
Document Type
Book Chapter
Publication Date
2014-01-01
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Abstract
Artificial nucleases have developed into powerful tools for introducing precise genome modifications in a wide variety of species. In this chapter the authors provide detailed protocols for rapidly constructing zinc finger nucleases (ZFNs) and TALE nucleases (TALENs) and evaluating their activity for the targeted generation of InDels within the zebrafish genome.
Source
Methods Mol Biol. 2014;1101:267-303. doi: 10.1007/978-1-62703-721-1_13. Link to article on publisher's site
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DOI
10.1007/978-1-62703-721-1_13
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PubMed ID
24233786