Activation of CD4 cells by fibronectin and anti-CD3 antibody. A synergistic effect mediated by the VLA-5 fibronectin receptor complex
Matsuyama, Takami Yamada, Akira Kay, Jonathan Yamada, Kenneth M. Akiyama, Steven K. Schlossman, Stuart F. Morimoto, Chikao
Citations
Student Authors
Faculty Advisor
Academic Program
UMass Chan Affiliations
Document Type
Publication Date
Keywords
Antigens, CD29
Antigens, CD3
Antigens, Differentiation
Antigens, Differentiation, T-Lymphocyte
CD4-Positive T-Lymphocytes
Cells, Cultured
Electrophoresis, Gel, Two-Dimensional
Extracellular Matrix
Fibronectins
Humans
In Vitro Techniques
*Lymphocyte Activation
Receptors, Antigen, T-Cell
Receptors, Cytoadhesin
Receptors, Very Late Antigen
Immunology and Infectious Disease
Musculoskeletal Diseases
Rheumatology
Skin and Connective Tissue Diseases
Subject Area
Files
Embargo Expiration Date
Link to Full Text
Abstract
In this study, fibronectin synergized with anti-CD3 antibody to promote CD4 cell proliferation in a serum-free culture system. The cell-adhesive domain plus additional regions of the fibronectin molecule are involved in this synergy. Anti4B4(CDw29) antibody blocked the activation of CD4 cells in this system. Furthermore, it is the VLA-5 protein within the set of molecules recognized by anti-4B4 that serves as a fibronectin receptor on the CD4 lymphocytes. The VLA-5 fibronectin receptor was mainly expressed on CD4+ CD45R-CDw29+ cells and may in part contribute to the unique function of these cells.
Source
J Exp Med. 1989 Oct 1;170(4):1133-48.
Year of Medical School at Time of Visit
Sponsors
Dates of Travel
DOI
Permanent Link to this Item
PubMed ID
Other Identifiers
Notes
At the time of publication, Jonathan Kay was not yet affiliated with the University of Massachusetts Medical School.