Preparation of early Drosophila embryonic fillets (before cuticle deposition)
Ramachandran, Preethi Budnik, Vivian
Ramachandran, Preethi
Budnik, Vivian
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Authors
Student Authors
Preethi Ramachandran
Faculty Advisor
Academic Program
Neuroscience
UMass Chan Affiliations
Document Type
Journal Article
Publication Date
2010-10-01
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Abstract
This article describes the dissection of early Drosophila embryos to generate flat or fillet preparations. For the procedure, a modified chamber or well is designed using glass slides and sealant. Embryos are dissected by removing the chorionic (outer) membrane (performed outside the chamber) and then removing the vitelline (inner) membrane (performed inside the chamber and under saline).
Source
Cold Spring Harb Protoc. 2010 Oct 1;2010(10):pdb.prot5497. doi: 10.1101/pdb.prot5497. Link to article on publisher's site.
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DOI
10.1101/pdb.prot5497
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PubMed ID
20889694