Quantitation of Dam methyltransferase in Escherichia coli
Boye, Erik ; Marinus, Martin G. ; Lobner-Olesen, Anders
Boye, Erik
Marinus, Martin G.
Lobner-Olesen, Anders
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UMass Chan Affiliations
Document Type
Journal Article
Publication Date
1992-03-01
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Abstract
An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employed to detect and quantitate the enzyme in immunoblots. A wild-type, rapidly growing E. coli cell (doubling time = 30 min) was found to contain about 130 molecules of Dam methyltransferase.
Source
J Bacteriol. 1992 Mar;174(5):1682-5.
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DOI
10.1128/jb.174.5.1682-1685.1992
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PubMed ID
1537808