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Regulated expression of the Escherichia coli dam gene

Calmann, Melissa A.
Marinus, Martin G.
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Journal Article
Publication Date
2003-08-05
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Abstract

Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease.

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J Bacteriol. 2003 Aug;185(16):5012-4.

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DOI
10.1128/JB.185.16.5012-5014.2003
PubMed ID
12897023
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