Regulated expression of the Escherichia coli dam gene
Calmann, Melissa A. ; Marinus, Martin G.
Calmann, Melissa A.
Marinus, Martin G.
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Journal Article
Publication Date
2003-08-05
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Abstract
Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease.
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J Bacteriol. 2003 Aug;185(16):5012-4.
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DOI
10.1128/JB.185.16.5012-5014.2003
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PubMed ID
12897023