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Biochemistry and Molecular Biology

Walhout, Albertha J M
; 
Boulton, Simon J.
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Walhout, Albertha J M
Boulton, Simon J.
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UMass Chan Affiliations
Program in Molecular Medicine
 Program in Gene Function and Expression
Document Type
Book Chapter
Publication Date
2006-02-13
Keywords
Caenorhabditis elegans
 Caenorhabditis elegans Proteins
 Protein Interaction Mapping
 Two-Hybrid System Techniques
 Tandem Mass Spectrometry
 Genetics and Genomics
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UMass Chan Faculty and Researcher Publications
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Abstract

Several forward and reverse proteomic approaches are available that can be used to identify interaction partners for a protein of interest. Here we provide methods for identifying interacting partners by the yeast two-hybrid system (a reverse proteomic method) and by tandem immuno-affinity purification of protein complexes combined with mass spectrometry (a forward proteomic method).

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Walhout, A.J.M. and Boulton, S.J. Biochemistry and molecular biology (February 13, 2006), WormBook, ed. The C. elegans Research Community, WormBook, http://dx.doi.org/110.1895/wormbook.1.86.1, http://www.wormbook.org.

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110.1895/wormbook.1.86.1
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https://hdl.handle.net/20.500.14038/43888
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