Macrophage interactions with laminin: PMA selectively induces the adherence and spreading of mouse macrophages on a laminin substratum

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dc.contributor.authorMercurio, Arthur M.
dc.contributor.authorShaw, Leslie M.
dc.contributor.departmentDepartment of Cancer Biology
dc.date2022-08-11T08:08:01.000
dc.date.accessioned2022-08-23T15:39:34Z
dc.date.available2022-08-23T15:39:34Z
dc.date.issued1988-11-01
dc.date.submitted2010-11-07
dc.description.abstractThe ability of thioglycollate (TG)-elicited mouse peritoneal macrophages to adhere to a laminin substratum has been studied. These cells do not adhere to laminin-coated (20 micrograms/ml) surfaces, but the addition of phorbol myristate acetate (PMA; 50 ng/ml) results in their rapid adherence and spreading on this substratum. TG-elicited and PMA-activated macrophages, however, can bind soluble laminin. Macrophages adhere to fibronectin-coated surfaces and tissue culture plastic without PMA stimulation, and PMA does not increase the number of cells that adhere to these surfaces. The predominant surface proteins that bind specifically to laminin-Sepharose exhibit an Mr of 67 and 36 kD, but the expression of these proteins does not increase after PMA stimulation. Laminin receptor antibodies immunoprecipitate the 67-kD protein from radiolabled surface lysates and are capable of blocking macrophage adherence to a laminin substratum. Indirect immunofluorescence microscopy indicates that PMA stimulation does not increase receptor expression, but that it may induce the aggregation of the receptor on the cell surface. PMA stimulation also promotes macrophage spreading and induces a reorganization of the actin cytoskeleton. Taken together, these data indicate the mechanism by which PMA promotes macrophage adherence to laminin does not involve increased 67-kD receptor surface expression, but that it is related to the changes in cytoskeletal and receptor surface organization that occur in response to PMA stimulation.
dc.identifier.citationJ Cell Biol. 1988 Nov;107(5):1873-80. <a href="http://dx.doi.org10.1083/jcb.107.5.1881">Link to article on publisher's website</a>
dc.identifier.contextkey1633336
dc.identifier.doi10.1083/jcb.107.5.1881
dc.identifier.issn0021-9525 (Linking)
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/cancerbiology_pp/111
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1111&amp;context=cancerbiology_pp&amp;unstamped=1
dc.identifier.pmid2972733
dc.identifier.submissionpathcancerbiology_pp/111
dc.identifier.urihttps://hdl.handle.net/20.500.14038/26202
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=2972733&dopt=Abstract">Link to Article in PubMed</a>
dc.source.issue5
dc.source.journaltitleThe Journal of cell biology
dc.source.pages1873-80
dc.source.volume107
dc.subjectAnimals
dc.subjectCell Adhesion
dc.subjectCell Movement
dc.subjectCytoskeleton
dc.subjectElectrophoresis, Polyacrylamide Gel
dc.subjectExtracellular Matrix
dc.subjectFemale
dc.subjectFibronectins
dc.subjectLaminin
dc.subjectMacrophages
dc.subjectMembrane Proteins
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectMicroscopy, Fluorescence
dc.subjectPrecipitin Tests
dc.subjectReceptors, Immunologic
dc.subjectReceptors, Laminin
dc.subjectTetradecanoylphorbol Acetate
dc.subjectCancer Biology
dc.subjectNeoplasms
dc.titleMacrophage interactions with laminin: PMA selectively induces the adherence and spreading of mouse macrophages on a laminin substratum
dc.typeJournal Article
dspace.entity.typePublication
html.description.abstract<p>The ability of thioglycollate (TG)-elicited mouse peritoneal macrophages to adhere to a laminin substratum has been studied. These cells do not adhere to laminin-coated (20 micrograms/ml) surfaces, but the addition of phorbol myristate acetate (PMA; 50 ng/ml) results in their rapid adherence and spreading on this substratum. TG-elicited and PMA-activated macrophages, however, can bind soluble laminin. Macrophages adhere to fibronectin-coated surfaces and tissue culture plastic without PMA stimulation, and PMA does not increase the number of cells that adhere to these surfaces. The predominant surface proteins that bind specifically to laminin-Sepharose exhibit an Mr of 67 and 36 kD, but the expression of these proteins does not increase after PMA stimulation. Laminin receptor antibodies immunoprecipitate the 67-kD protein from radiolabled surface lysates and are capable of blocking macrophage adherence to a laminin substratum. Indirect immunofluorescence microscopy indicates that PMA stimulation does not increase receptor expression, but that it may induce the aggregation of the receptor on the cell surface. PMA stimulation also promotes macrophage spreading and induces a reorganization of the actin cytoskeleton. Taken together, these data indicate the mechanism by which PMA promotes macrophage adherence to laminin does not involve increased 67-kD receptor surface expression, but that it is related to the changes in cytoskeletal and receptor surface organization that occur in response to PMA stimulation.</p>
refterms.dateFOA2022-08-23T15:39:34Z
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