Opioid medication use and blood DNA methylation: epigenome-wide association meta-analysis
Lee, Mikyeong Joehanes, Roby McCartney, Daniel L Kho, Minjung Hüls, Anke Wyss, Annah B Liu, Chunyu Walker, Rosie M R Kardia, Sharon L Wingo, Thomas S
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Joehanes, Roby
McCartney, Daniel L
Kho, Minjung
Hüls, Anke
Wyss, Annah B
Liu, Chunyu
Walker, Rosie M
R Kardia, Sharon L
Wingo, Thomas S
Burkholder, Adam
Ma, Jiantao
Campbell, Archie
Wingo, Aliza P
Huan, Tianxiao
Sikdar, Sinjini
Keshawarz, Amena
Bennett, David A
Smith, Jennifer A
Evans, Kathryn L
Levy, Daniel
London, Stephanie J
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UMass Chan Affiliations
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Abstract
Aim: To identify differential methylation related to prescribed opioid use. Methods: This study examined whether blood DNA methylation, measured using Illumina arrays, differs by recent opioid medication use in four population-based cohorts. We meta-analyzed results (282 users; 10,560 nonusers) using inverse-variance weighting. Results: Differential methylation (false discovery rate <0.05) was observed at six CpGs annotated to the following genes: KIAA0226, CPLX2, TDRP, RNF38, TTC23 and GPR179. Integrative epigenomic analyses linked implicated loci to regulatory elements in blood and/or brain. Additionally, 74 CpGs were differentially methylated in males or females. Methylation at significant CpGs correlated with gene expression in blood and/or brain. Conclusion: This study identified DNA methylation related to opioid medication use in general populations. The results could inform the development of blood methylation biomarkers of opioid use.
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Lee M, Joehanes R, McCartney DL, Kho M, Hüls A, Wyss AB, Liu C, Walker RM, R Kardia SL, Wingo TS, Burkholder A, Ma J, Campbell A, Wingo AP, Huan T, Sikdar S, Keshawarz A, Bennett DA, Smith JA, Evans KL, Levy D, London SJ. Opioid medication use and blood DNA methylation: epigenome-wide association meta-analysis. Epigenomics. 2022 Dec;14(23):1479-1492. doi: 10.2217/epi-2022-0353. Epub 2023 Jan 26. PMID: 36700736; PMCID: PMC9979153.