Target accessibility dictates the potency of human RISC
Brown, Kirk M. Chu, Chia-ying Rana, Tariq M.
Brown, Kirk M.
Chu, Chia-ying
Rana, Tariq M.
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Journal Article
Publication Date
2005-04-27
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Abstract
In this report, we examined the effect of increased target site access on activated human RNA-induced silencing complex (RISC(*)) catalysis. Kinetic studies revealed that siRNA-programmed RISC(*) cleaved target RNA with higher efficiencies when target site access was increased. These results provide evidence that target site access is linked to RISC(*) catalysis.
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Nat Struct Mol Biol. 2005 May;12(5):469-70. Epub 2005 Apr 24. Link to article on publisher's site
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DOI
10.1038/nsmb931
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PubMed ID
15852021