CSF-1 regulation of osteoclastogenesis for tooth eruption
Wise, Gary E. ; Yao, S. ; Odgren, Paul R. ; Pan, Feng
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Keywords
Dental Sac
Down-Regulation
Gene Expression Regulation, Developmental
Glycoproteins
Immunoenzyme Techniques
Macrophage Colony-Stimulating Factor
Osteoclasts
Osteoprotegerin
RNA, Small Interfering
Rats
Rats, Mutant Strains
Receptors, Cytoplasmic and Nuclear
Receptors, Tumor Necrosis Factor
Reverse Transcriptase Polymerase Chain Reaction
Tooth Eruption
Transfection
Cell Biology
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Abstract
The dental follicle regulates the alveolar bone resorption needed for tooth eruption. In the rat first mandibular molar, a decrease in the expression of osteoprotegerin (OPG) in the dental follicle at day 3 enables the osteoclastogenesis needed for eruption to occur. Because colony-stimulating factor-1 (CSF-1) is maximally expressed in the dental follicle at day 3, it was hypothesized that CSF-1 down-regulates OPG gene expression in the dental follicle in vivo. To test this, we compared the expression of OPG in osteopetrotic toothless (tl/tl) rats deficient in CSF-1 with expression in their normal littermates for given ages. OPG gene expression was found to be higher in the dental follicle of the tl/tl mutants than in normals. Transfecting short interfering RNA specific for CSF-1 mRNA into dental follicle cells resulted in an up-regulation of OPG expression. Thus, these studies support our hypothesis that the down-regulation of OPG needed for tooth eruption is mediated by CSF-1.
Source
J Dent Res. 2005 Sep;84(9):837-41.