A reference human induced pluripotent stem cell line for large-scale collaborative studies
Pantazis, Caroline B ; Yang, Andrian ; Lara, Erika ; McDonough, Justin A ; Blauwendraat, Cornelis ; Peng, Lirong ; Oguro, Hideyuki ; Kanaujiya, Jitendra ; Zou, Jizhong ; Sebesta, David ... show 10 more
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Authors
Yang, Andrian
Lara, Erika
McDonough, Justin A
Blauwendraat, Cornelis
Peng, Lirong
Oguro, Hideyuki
Kanaujiya, Jitendra
Zou, Jizhong
Sebesta, David
Pratt, Gretchen
Cross, Erin
Blockwick, Jeffrey
Buxton, Philip
Kinner-Bibeau, Lauren
Medura, Constance
Tompkins, Christopher
Hughes, Stephen
Santiana, Marianita
Faghri, Faraz
Nalls, Mike A
Vitale, Daniel
Ballard, Shannon
Qi, Yue A
Ramos, Daniel M
Anderson, Kailyn M
Stadler, Julia
Narayan, Priyanka
Papademetriou, Jason
Reilly, Luke
Nelson, Matthew P
Aggarwal, Sanya
Rosen, Leah U
Kirwan, Peter
Pisupati, Venkat
Coon, Steven L
Scholz, Sonja W
Priebe, Theresa
Öttl, Miriam
Dong, Jian
Meijer, Marieke
Janssen, Lara J M
Lourenco, Vanessa S
van der Kant, Rik
Crusius, Dennis
Paquet, Dominik
Raulin, Ana-Caroline
Bu, Guojun
Held, Aaron
Wainger, Brian J
Gabriele, Rebecca M C
Casey, Jackie M
Wray, Selina
Abu-Bonsrah, Dad
Parish, Clare L
Beccari, Melinda S
Cleveland, Don W
Li, Emmy
Rose, Indigo V L
Kampmann, Martin
Calatayud Aristoy, Carles
Verstreken, Patrik
Heinrich, Laurin
Chen, Max Y
Schüle, Birgitt
Dou, Dan
Holzbaur, Erika L F
Zanellati, Maria Clara
Basundra, Richa
Deshmukh, Mohanish
Cohen, Sarah
Khanna, Richa
Raman, Malavika
Nevin, Zachary S
Matia, Madeline
Van Lent, Jonas
Timmerman, Vincent
Conklin, Bruce R
Johnson Chase, Katherine
Zhang, Ke
Funes, Salome
Bosco, Daryl A
Erlebach, Lena
Welzer, Marc
Kronenberg-Versteeg, Deborah
Lyu, Guochang
Arenas, Ernest
Coccia, Elena
Sarrafha, Lily
Ahfeldt, Tim
Marioni, John C
Skarnes, William C
Cookson, Mark R
Ward, Michael E
Merkle, Florian T
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UMass Chan Affiliations
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Abstract
Human induced pluripotent stem cell (iPSC) lines are a powerful tool for studying development and disease, but the considerable phenotypic variation between lines makes it challenging to replicate key findings and integrate data across research groups. To address this issue, we sub-cloned candidate human iPSC lines and deeply characterized their genetic properties using whole genome sequencing, their genomic stability upon CRISPR-Cas9-based gene editing, and their phenotypic properties including differentiation to commonly used cell types. These studies identified KOLF2.1J as an all-around well-performing iPSC line. We then shared KOLF2.1J with groups around the world who tested its performance in head-to-head comparisons with their own preferred iPSC lines across a diverse range of differentiation protocols and functional assays. On the strength of these findings, we have made KOLF2.1J and its gene-edited derivative clones readily accessible to promote the standardization required for large-scale collaborative science in the stem cell field.
Source
Pantazis CB, Yang A, Lara E, McDonough JA, Blauwendraat C, Peng L, Oguro H, Kanaujiya J, Zou J, Sebesta D, Pratt G, Cross E, Blockwick J, Buxton P, Kinner-Bibeau L, Medura C, Tompkins C, Hughes S, Santiana M, Faghri F, Nalls MA, Vitale D, Ballard S, Qi YA, Ramos DM, Anderson KM, Stadler J, Narayan P, Papademetriou J, Reilly L, Nelson MP, Aggarwal S, Rosen LU, Kirwan P, Pisupati V, Coon SL, Scholz SW, Priebe T, Öttl M, Dong J, Meijer M, Janssen LJM, Lourenco VS, van der Kant R, Crusius D, Paquet D, Raulin AC, Bu G, Held A, Wainger BJ, Gabriele RMC, Casey JM, Wray S, Abu-Bonsrah D, Parish CL, Beccari MS, Cleveland DW, Li E, Rose IVL, Kampmann M, Calatayud Aristoy C, Verstreken P, Heinrich L, Chen MY, Schüle B, Dou D, Holzbaur ELF, Zanellati MC, Basundra R, Deshmukh M, Cohen S, Khanna R, Raman M, Nevin ZS, Matia M, Van Lent J, Timmerman V, Conklin BR, Johnson Chase K, Zhang K, Funes S, Bosco DA, Erlebach L, Welzer M, Kronenberg-Versteeg D, Lyu G, Arenas E, Coccia E, Sarrafha L, Ahfeldt T, Marioni JC, Skarnes WC, Cookson MR, Ward ME, Merkle FT. A reference human induced pluripotent stem cell line for large-scale collaborative studies. Cell Stem Cell. 2022 Dec 1;29(12):1685-1702.e22. doi: 10.1016/j.stem.2022.11.004. PMID: 36459969; PMCID: PMC9782786.