Preparation of late Drosophila embryonic fillets
Ramachandran, Preethi Budnik, Vivian
Ramachandran, Preethi
Budnik, Vivian
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Authors
Student Authors
Preethi Ramachandran
Faculty Advisor
Academic Program
Neuroscience
UMass Chan Affiliations
Document Type
Journal Article
Publication Date
2010-10-05
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Abstract
Beyond the age of ~15 h (at 25 degrees C), Drosophila embryos become impenetrable to aqueous fixatives and staining methods as a result of cuticle deposition. This problem can be overcome using flat or fillet preparations, as described in this protocol.
Source
Cold Spring Harb Protoc. 2010 Oct 1;2010(10):pdb.prot5498. doi: 10.1101/pdb.prot5498.
Year of Medical School at Time of Visit
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DOI
10.1101/pdb.prot5498
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PubMed ID
20889695