Integration of single-cell transcriptomes and chromatin landscapes reveals regulatory programs driving pharyngeal organ development

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dc.contributor.authorMagaletta, Margaret E
dc.contributor.authorLobo, Macrina
dc.contributor.authorKernfeld, Eric M
dc.contributor.authorAliee, Hananeh
dc.contributor.authorHuey, Jack D
dc.contributor.authorParsons, Teagan J
dc.contributor.authorTheis, Fabian J
dc.contributor.authorMaehr, René
dc.contributor.departmentDiabetes Center of Excellenceen_US
dc.contributor.departmentMorningside Graduate School of Biomedical Sciencesen_US
dc.contributor.departmentProgram in Molecular Medicineen_US
dc.contributor.studentJack Huey
dc.date.accessioned2023-06-27T15:14:30Z
dc.date.available2023-06-27T15:14:30Z
dc.date.issued2022-01-24
dc.description.abstractMaldevelopment of the pharyngeal endoderm, an embryonic tissue critical for patterning of the pharyngeal region and ensuing organogenesis, ultimately contributes to several classes of human developmental syndromes and disorders. Such syndromes are characterized by a spectrum of phenotypes that currently cannot be fully explained by known mutations or genetic variants due to gaps in characterization of critical drivers of normal and dysfunctional development. Despite the disease-relevance of pharyngeal endoderm, we still lack a comprehensive and integrative view of the molecular basis and gene regulatory networks driving pharyngeal endoderm development. To close this gap, we apply transcriptomic and chromatin accessibility single-cell sequencing technologies to generate a multi-omic developmental resource spanning pharyngeal endoderm patterning to the emergence of organ-specific epithelia in the developing mouse embryo. We identify cell-type specific gene regulation, distill GRN models that define developing organ domains, and characterize the role of an immunodeficiency-associated forkhead box transcription factor.en_US
dc.identifier.citationMagaletta ME, Lobo M, Kernfeld EM, Aliee H, Huey JD, Parsons TJ, Theis FJ, Maehr R. Integration of single-cell transcriptomes and chromatin landscapes reveals regulatory programs driving pharyngeal organ development. Nat Commun. 2022 Jan 24;13(1):457. doi: 10.1038/s41467-022-28067-4. PMID: 35075189; PMCID: PMC8786836.en_US
dc.identifier.doi10.1038/s41467-022-28067-4en_US
dc.identifier.eissn2041-1723
dc.identifier.journalNature communications
dc.identifier.pmid35075189
dc.identifier.urihttps://hdl.handle.net/20.500.14038/52197
dc.language.isoenen_US
dc.relation.ispartofNature Communicationsen_US
dc.relation.urlhttps://doi.org/10.1038/s41467-022-28067-4en_US
dc.rightsOpen Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/. © The Author(s) 2022en_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.source.beginpage457
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryEngland
dc.source.endpage
dc.source.issue1
dc.source.journaltitleNature communications
dc.source.volume13
dc.subjectDifferentiationen_US
dc.subjectEpigenetics analysisen_US
dc.subjectGene expression analysisen_US
dc.subjectOrganogenesisen_US
dc.subjectPattern formationen_US
dc.titleIntegration of single-cell transcriptomes and chromatin landscapes reveals regulatory programs driving pharyngeal organ developmenten_US
dc.typeJournal Articleen_US
dspace.entity.typePublication
refterms.dateFOA2023-06-27T15:14:32Z
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