Thumbnail Image
Publication

Drosophila Model of Leishmania amazonensis Infection

Okuda, Kendi
; 
Silverman, Neal
Citations
Altmetric:
Authors
Okuda, Kendi
Silverman, Neal
Student Authors
Faculty Advisor
Academic Program
UMass Chan Affiliations
Division of Infectious Diseases and Immunology, Department of Medicine
Document Type
Journal Article
Publication Date
2017-12-21
Keywords
Amastigote
 Axenic amastigote
 Drosophila melanogaster
 Hemocytes
 Leishmania
 Phagocytosis
 Plasmatocytes
 RNAi screen
 Immunology and Infectious Disease
 Research Methods in Life Sciences
Subject Area
Collections
UMass Chan Faculty and Researcher Publications
Show all metadata
Files
Thumbnail Image
Bio_protocol2640.pdf
Adobe PDF, 4.86 MB
Embargo Expiration Date
Link to Full Text
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738924/
Abstract

This protocol describes how to generate and harvest antibody-free L. amazonensis amastigotes, and how to infect adult Drosophila melanogaster with these parasites. This model recapitulates key aspects of the interactions between Leishmania amastigotes and animal phagocytes.

Source

Bio Protoc. 2017 Dec 5;7(23). doi: 10.21769/BioProtoc.2640. Link to article on publisher's site

Year of Medical School at Time of Visit
Sponsors
Dates of Travel
DOI
10.21769/BioProtoc.2640
Permanent Link to this Item
https://hdl.handle.net/20.500.14038/40519
PubMed ID
29276726
Other Identifiers
Notes
Funding and Acknowledgements
Corresponding Author
Related Resources

Link to Article in PubMed

Related Resources
Repository Citation
Rights
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.
Distribution License