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A simple method for improving the specificity of anti-methyl histone antibodies
Connor, Caroline M. ; Cheung, Iris ; Simon, Andrew ; Jakovcevski, Mira ; Weng, Zhiping ; Akbarian, Schahram
Connor, Caroline M.
Cheung, Iris
Simon, Andrew
Jakovcevski, Mira
Weng, Zhiping
Akbarian, Schahram
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Caroline Connor
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Journal Article
Publication Date
2010-07-01
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Abstract
Antibodies differentiating between the mono-, di- and trimethylated forms of specific histone lysine residues are a critical tool in epigenome research, but show variable specificity, potentially limiting comparisons across studies and between samples. Using trimethyl histone H3 lysine 4 (H3K4me3)-a mark enriched at transcription start sites (TSS) of active genes-as an example, we describe how simple co-incubation with synthetic peptide of the K4me2 modification leads to increased specificity for K4me3 and a much sharper peak distribution proximal to TSS following chromatin immunoprecipitation and massively parallel sequencing (ChIP-Seq).
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Epigenetics. 2010 Jul 1;5(5):392-5. Epub 2010 Jul 1. Link to article on publisher's website
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DOI
10.4161/epi.5.5.11874
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20458167
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This is an open access article licensed under a under a Creative Commons Attribution-NonCommercial 3.0 Unported License.