Publication

Synthesis of pCpCpA-3'-NH-phenylalanine as a ribosomal substrate

Zhang, Biliang
Zhang, Lei
Sun, Lele
Cui, Zhiyong
Citations
Altmetric:
Student Authors
Faculty Advisor
Academic Program
Document Type
Journal Article
Publication Date
2002-10-12
Keywords
Subject Area
Embargo Expiration Date
Abstract

[reaction: see text] The trinucleotide cytidylyl(3'-->5'phosphoryl)cytidylyl(3'-->5'phosphoryl)-3'-deoxy-3'-(L-ph enylalanyl) amido adenosine (CpCpA-NH-Phe) was synthesized by phosphoramidite chemistry from 3'-amino-3'-deoxyadenosine as the ribosomal substrate. The 3'-amino-3'-deoxyadenosine was first converted to 3'-(N-tert-butyloxycarbonyl-L-phenylalanine)amido-3'-deoxy-6-N,6-N,2'-O-tr ibenzoyl-adenosine and then coupled with cytidine phosphoramidite to produce the fully protected CpCpA-NH-Phe-Boc. The title product was obtained after removing all protection groups and then radiolabeled with (32)P to yield pCpCpA-NH-Phe, which demonstrated high activity for the peptidyl transferase reaction in the ribosome.

Source

Org Lett. 2002 Oct 17;4(21):3615-8.

Year of Medical School at Time of Visit
Sponsors
Dates of Travel
DOI
10.1021/ol026560f
PubMed ID
12375901
Other Identifiers
Notes
Funding and Acknowledgements
Corresponding Author
Related Resources
Related Resources
Repository Citation
Rights
Distribution License