Isolation and characterization of Dam+ revertants and suppressor mutations that modify secondary phenotypes of dam-3 strains of Escherichia coli K-12
McGraw, Bonnie R. Marinus, Martin G.
Citations
Authors
Student Authors
Faculty Advisor
Academic Program
UMass Chan Affiliations
Document Type
Publication Date
Subject Area
Embargo Expiration Date
Link to Full Text
Abstract
Bacteria mutant in the dam (DNA adenine methylation) gene and in either recA or recB or recC genes are inviable (Virm- phenotype). From crosses between dam-3 bacteria and recA1 or recB21 recC22 strains, Vrm+ recombinants were recovered. Among these recombinants, Dam+ revertants were present which did not show the phenotypes normally associated with dam-3 bacteria. Three classes of indirectly suppressed strains (dam-3 genotype) were also recovered which showed alterations in the secondary phenotypes normally associated with dam-3 bacteria. These strains contained a second unlinked mutation in either mutL or mutS or sin. In addition, mutation in either sbcA or sbcB supresses the Vrm- phenotype of dam-3 recB21 recC22 strains.
Source
Mol Gen Genet. 1980;178(2):309-15.