Single cell transcriptomic profiling of large intestinal enteroendocrine cells in mice - Identification of selective stimuli for insulin-like peptide-5 and glucagon-like peptide-1 co-expressing cells

Show simplified metadata
dc.contributor.authorBilling, Lawrence J.
dc.contributor.authorLarraufie, Pierre
dc.contributor.authorLewis, Jo
dc.contributor.authorLeiter, Andrew B.
dc.contributor.authorLi, Hui Joyce
dc.contributor.authorLam, Brian
dc.contributor.authorYeo, Giles Sh.
dc.contributor.authorGoldspink, Deborah A.
dc.contributor.authorKay, Richard G.
dc.contributor.authorGribble, Fiona M.
dc.contributor.authorReimann, Frank
dc.contributor.departmentDivision of Gastroenterology, Department of Medicine
dc.date2022-08-11T08:09:54.000
dc.date.accessioned2022-08-23T16:48:29Z
dc.date.available2022-08-23T16:48:29Z
dc.date.issued2019-11-01
dc.date.submitted2019-12-12
dc.description.abstractOBJECTIVE: Enteroendocrine cells (EECs) of the large intestine, found scattered in the epithelial layer, are known to express different hormones, with at least partial co-expression of different hormones in the same cell. Here we aimed to categorize colonic EECs and to identify possible targets for selective recruitment of hormones. METHODS: Single cell RNA-sequencing of sorted enteroendocrine cells, using NeuroD1-Cre x Rosa26-EYFP mice, was used to cluster EECs from the colon and rectum according to their transcriptome. G-protein coupled receptors differentially expressed across clusters were identified, and, as a proof of principle, agonists of Agtr1a and Avpr1b were tested as candidate EEC secretagogues in vitro and in vivo. RESULTS: EECs from the large intestine separated into 7 clear clusters, 4 expressing higher levels of Tph1 (enzyme required for serotonin (5-HT) synthesis; enterochromaffin cells), 2 enriched for Gcg (encoding glucagon-like peptide-1, GLP-1, L-cells), and the 7th expressing somatostatin (D-cells). Restricted analysis of L-cells identified 4 L-cell sub-clusters, exhibiting differential expression of Gcg, Pyy (Peptide YY), Nts (neurotensin), Insl5 (insulin-like peptide 5), Cck (cholecystokinin), and Sct (secretin). Expression profiles of L- and enterochromaffin cells revealed the clustering to represent gradients along the crypt-surface (cell maturation) and proximal-distal gut axes. Distal colonic/rectal L-cells differentially expressed Agtr1a and the ligand angiotensin II was shown to selectively increase GLP-1 and PYY release in vitro and GLP-1 in vivo. CONCLUSION: EECs in the large intestine exhibit differential expression gradients along the crypt-surface and proximal-distal axes. Distal L-cells can be differentially stimulated by targeting receptors such as Agtr1a.
dc.identifier.citation<p>Mol Metab. 2019 Nov;29:158-169. doi: 10.1016/j.molmet.2019.09.001. Epub 2019 Sep 7. <a href="https://doi.org/10.1016/j.molmet.2019.09.001">Link to article on publisher's site</a></p>
dc.identifier.contextkey15986389
dc.identifier.doi10.1016/j.molmet.2019.09.001
dc.identifier.issn2212-8778 (Linking)
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/oapubs/4069
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5088&amp;context=oapubs&amp;unstamped=1
dc.identifier.pmid31668387
dc.identifier.submissionpathoapubs/4069
dc.identifier.urihttps://hdl.handle.net/20.500.14038/41282
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=31668387&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsCopyright 2019 The Authors. Published by Elsevier GmbH. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.source.journaltitleMolecular metabolism
dc.source.pages158-169
dc.source.volume29
dc.subjectEnteroendocrine cells
dc.subjectGlucagon-like peptide-1 (GLP-1)
dc.subjectInsulin-like peptide-5 (Insl5)
dc.subjectSerotonin (5-HT)
dc.subjectSingle cell RNA-sequencing
dc.subjectCell Biology
dc.subjectCellular and Molecular Physiology
dc.subjectDigestive System
dc.subjectGastroenterology
dc.subjectMolecular Biology
dc.titleSingle cell transcriptomic profiling of large intestinal enteroendocrine cells in mice - Identification of selective stimuli for insulin-like peptide-5 and glucagon-like peptide-1 co-expressing cells
dc.typeJournal Article
dspace.entity.typePublication
html.description.abstract<p>OBJECTIVE: Enteroendocrine cells (EECs) of the large intestine, found scattered in the epithelial layer, are known to express different hormones, with at least partial co-expression of different hormones in the same cell. Here we aimed to categorize colonic EECs and to identify possible targets for selective recruitment of hormones.</p> <p>METHODS: Single cell RNA-sequencing of sorted enteroendocrine cells, using NeuroD1-Cre x Rosa26-EYFP mice, was used to cluster EECs from the colon and rectum according to their transcriptome. G-protein coupled receptors differentially expressed across clusters were identified, and, as a proof of principle, agonists of Agtr1a and Avpr1b were tested as candidate EEC secretagogues in vitro and in vivo.</p> <p>RESULTS: EECs from the large intestine separated into 7 clear clusters, 4 expressing higher levels of Tph1 (enzyme required for serotonin (5-HT) synthesis; enterochromaffin cells), 2 enriched for Gcg (encoding glucagon-like peptide-1, GLP-1, L-cells), and the 7th expressing somatostatin (D-cells). Restricted analysis of L-cells identified 4 L-cell sub-clusters, exhibiting differential expression of Gcg, Pyy (Peptide YY), Nts (neurotensin), Insl5 (insulin-like peptide 5), Cck (cholecystokinin), and Sct (secretin). Expression profiles of L- and enterochromaffin cells revealed the clustering to represent gradients along the crypt-surface (cell maturation) and proximal-distal gut axes. Distal colonic/rectal L-cells differentially expressed Agtr1a and the ligand angiotensin II was shown to selectively increase GLP-1 and PYY release in vitro and GLP-1 in vivo.</p> <p>CONCLUSION: EECs in the large intestine exhibit differential expression gradients along the crypt-surface and proximal-distal axes. Distal L-cells can be differentially stimulated by targeting receptors such as Agtr1a.</p>
refterms.dateFOA2022-08-23T16:48:29Z
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
1_s2.0_S2212877819307628_main.pdf
Size:
2.62 MB
Format:
Adobe Portable Document Format
Download
Collections
UMass Chan Faculty and Researcher Publications