Loading...
Thumbnail Image
Publication

Ca2+ syntillas, miniature Ca2+ release events in terminals of hypothalamic neurons, are increased in frequency by depolarization in the absence of Ca2+ influx

De Crescenzo, Valerie
ZhuGe, Ronghua
Velazquez-Marrero, Cristina M.
Lifshitz, Lawrence M.
Custer, Edward E.
Carmichael, Jeffrey
Lai, F. Anthony
Tuft, Richard A.
Fogarty, Kevin E.
Lemos, Jose R.
... show 1 more
Embargo Expiration Date
Link to Full Text
Abstract

Localized, brief Ca2+ transients (Ca2+ syntillas) caused by release from intracellular stores were found in isolated nerve terminals from magnocellular hypothalamic neurons and examined quantitatively using a signal mass approach to Ca2+ imaging. Ca2+ syntillas (scintilla, L., spark, from a synaptic structure, a nerve terminal) are caused by release of approximately 250,000 Ca ions on average by a Ca2+ flux lasting on the order of tens of milliseconds and occur spontaneously at a membrane potential of -80 mV. Syntillas are unaffected by removal of extracellular Ca2+, are mediated by ryanodine receptors (RyRs) and are increased in frequency, in the absence of extracellular Ca2+, by physiological levels of depolarization. This represents the first direct demonstration of mobilization of Ca2+ from intracellular stores in neurons by depolarization without Ca2+ influx. The regulation of syntillas by depolarization provides a new link between neuronal activity and cytosolic [Ca2+] in nerve terminals.

Source

J Neurosci. 2004 Feb 4;24(5):1226-35. Link to article on publisher's site

Year of Medical School at Time of Visit
Sponsors
Dates of Travel
DOI
10.1523/JNEUROSCI.4286-03.2004
PubMed ID
14762141
Other Identifiers
Notes

Co-author Cristina M. Velazquez-Marrero is a student in the Neuroscience program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.

Funding and Acknowledgements
Corresponding Author
Related Resources
Related Resources
Repository Citation
Rights
Distribution License