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dc.contributor.authorNishinaga, Hiromi
dc.contributor.authorTakahashi-Niki, Kazuko
dc.contributor.authorTaira, Takahiro
dc.contributor.authorAndreadis, Athena
dc.contributor.authorIguchi-Ariga, Sanai M. M.
dc.contributor.authorAriga, Hiroyoshi
dc.date2022-08-11T08:07:57.000
dc.date.accessioned2022-08-23T15:37:14Z
dc.date.available2022-08-23T15:37:14Z
dc.date.issued2005-08-24
dc.date.submitted2010-09-30
dc.identifier.citationNeurosci Lett. 2005 Dec 16;390(1):54-9. <a href="http://dx.doi.org/10.1016/j.neulet.2005.07.053">Link to article on publisher's site</a>
dc.identifier.issn0304-3940 (Linking)
dc.identifier.doi10.1016/j.neulet.2005.07.053
dc.identifier.pmid16115732
dc.identifier.urihttp://hdl.handle.net/20.500.14038/25675
dc.description.abstractDJ-1 is a novel oncogene and a causative gene for the familial form of Parkinson's disease (PD). DJ-1 has been shown to play roles in anti-oxidative stress by eliminating reactive oxygen species and in transcriptional regulation of genes. Loss of these functions of DJ-1 is thought to trigger the onset of PD. In this study, to identify genes for which expressions are regulated by DJ-1, DNA microarray analyses were carried out using two mouse NIH3T3 cell lines, DJ-1-knockdown cells and cells harboring an exogenously added L 166 P DJ-1 mutant found in PD patients. In both cell lines, drastic changes in expressions of genes, including genes related to stress, apoptosis, oxidative stress and neurotoxicity, were observed and changes in expressions were confirmed by RT-PCR. Of the genes identified, expression level of the extracellular superoxide dismutase (SOD 3) gene was found to decrease in DJ-1-knockdown cells, while expressions of SOD 1 and SOD 2 genes did not change. Furthermore, expression of the tau gene, a gene whose product gives cells neurotoxicity by aggregation, was found to increase at its promoter level in L 166 P DJ-1 cells. These findings suggest that DJ-1 regulates expressions of genes for which functions are thought to be related to cell death or neurodegeneration.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=16115732&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1016/j.neulet.2005.07.053
dc.subjectAmino Acid Substitution
dc.subjectAnimals
dc.subjectGene Expression Regulation
dc.subjectGene Silencing
dc.subjectMice
dc.subjectMutation
dc.subjectNIH 3T3 Cells
dc.subjectOncogene Proteins
dc.subjectStructure-Activity Relationship
dc.subjectSuperoxide Dismutase
dc.subjecttau Proteins
dc.subjectCell Biology
dc.titleExpression profiles of genes in DJ-1-knockdown and L 166 P DJ-1 mutant cells
dc.typeJournal Article
dc.source.journaltitleNeuroscience letters
dc.source.volume390
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/andreadis/11
dc.identifier.contextkey1587268
html.description.abstract<p>DJ-1 is a novel oncogene and a causative gene for the familial form of Parkinson's disease (PD). DJ-1 has been shown to play roles in anti-oxidative stress by eliminating reactive oxygen species and in transcriptional regulation of genes. Loss of these functions of DJ-1 is thought to trigger the onset of PD. In this study, to identify genes for which expressions are regulated by DJ-1, DNA microarray analyses were carried out using two mouse NIH3T3 cell lines, DJ-1-knockdown cells and cells harboring an exogenously added L 166 P DJ-1 mutant found in PD patients. In both cell lines, drastic changes in expressions of genes, including genes related to stress, apoptosis, oxidative stress and neurotoxicity, were observed and changes in expressions were confirmed by RT-PCR. Of the genes identified, expression level of the extracellular superoxide dismutase (SOD 3) gene was found to decrease in DJ-1-knockdown cells, while expressions of SOD 1 and SOD 2 genes did not change. Furthermore, expression of the tau gene, a gene whose product gives cells neurotoxicity by aggregation, was found to increase at its promoter level in L 166 P DJ-1 cells. These findings suggest that DJ-1 regulates expressions of genes for which functions are thought to be related to cell death or neurodegeneration.</p>
dc.identifier.submissionpathandreadis/11
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages54-9


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