Identification, expression analysis, genomic organization and cellular location of a novel protein with a RhoGEF domain
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Publisher version
Document Type
ArticlePublication Date
2005-09-07Keywords
Amino Acid SequenceAnimals
Blood Proteins
Blotting, Northern
Blotting, Western
Brain
Carrier Proteins
Cell Line
Cell Line, Tumor
Cloning, MolecularCytoplasmDNA, ComplementaryExons*Gene Expression ProfilingGuanine Nucleotide Exchange FactorsHumansImmunohistochemistryImmunoprecipitationIntronsMaleMiceMolecular Sequence DataPhosphoproteinsProtein BindingRNA, MessengerRatsReverse Transcriptase Polymerase Chain ReactionSaccharomyces cerevisiaeSequence AlignmentSequence Analysis, DNASequence Homology, Amino AcidTwo-Hybrid System Techniquestau ProteinsCell Biology
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In this study we describe the identification and characterization of a novel cytosolic protein of the guanine exchange factor (GEF) family. The human cDNA corresponds to predicted human protein FLJ00128/FLJ10357 located on chromosome 14q11.2. The deduced protein sequence contains in its C-terminus a RhoGEF domain followed by a pleckstrin domain. Its N-terminus, central region and RhoGEF/pleckstrin domain are homologous to the recently identified zebrafish Quattro protein, which is involved in morphogenetic movements mediated by the actin cytoskeleton. Based on the homology of our protein's RhoGEF domain to the RhoGEF domains of Trio, Duo and Duet and its homology with Quattro, we named it Solo. The Solo mRNA is ubiquitously expressed but enriched in brain, its expression peaks perinatally and it undergoes extensive alternative splicing. In both myoblasts and neuroblastoma cells, the Solo protein is concentrated around the nucleus.Source
Gene. 2005 Oct 10;359:63-72. Link to article on publisher's siteDOI
10.1016/j.gene.2005.06.025Permanent Link to this Item
http://hdl.handle.net/20.500.14038/25677PubMed ID
16143467Related Resources
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