Identification, expression analysis, genomic organization and cellular location of a novel protein with a RhoGEF domain
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Authors
Tse, Sze-WahBroderick, Jennifer A.
Wei, Mei-Ling
Luo, Min-hua
Smith, Deborah
McCaffery, Peter J.
Stamm, Stefan
Andreadis, Athena
Document Type
Journal ArticlePublication Date
2005-09-07Keywords
Amino Acid SequenceAnimals
Blood Proteins
Blotting, Northern
Blotting, Western
Brain
Carrier Proteins
Cell Line
Cell Line, Tumor
Cloning, Molecular
Cytoplasm
DNA, Complementary
Exons
*Gene Expression Profiling
Guanine Nucleotide Exchange Factors
Humans
Immunohistochemistry
Immunoprecipitation
Introns
Male
Mice
Molecular Sequence Data
Phosphoproteins
Protein Binding
RNA, Messenger
Rats
Reverse Transcriptase Polymerase Chain Reaction
Saccharomyces cerevisiae
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Two-Hybrid System Techniques
tau Proteins
Cell Biology
Metadata
Show full item recordAbstract
In this study we describe the identification and characterization of a novel cytosolic protein of the guanine exchange factor (GEF) family. The human cDNA corresponds to predicted human protein FLJ00128/FLJ10357 located on chromosome 14q11.2. The deduced protein sequence contains in its C-terminus a RhoGEF domain followed by a pleckstrin domain. Its N-terminus, central region and RhoGEF/pleckstrin domain are homologous to the recently identified zebrafish Quattro protein, which is involved in morphogenetic movements mediated by the actin cytoskeleton. Based on the homology of our protein's RhoGEF domain to the RhoGEF domains of Trio, Duo and Duet and its homology with Quattro, we named it Solo. The Solo mRNA is ubiquitously expressed but enriched in brain, its expression peaks perinatally and it undergoes extensive alternative splicing. In both myoblasts and neuroblastoma cells, the Solo protein is concentrated around the nucleus.Source
Gene. 2005 Oct 10;359:63-72. Link to article on publisher's siteDOI
10.1016/j.gene.2005.06.025Permanent Link to this Item
http://hdl.handle.net/20.500.14038/25677PubMed ID
16143467Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/j.gene.2005.06.025
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