Show simple item record

dc.contributor.authorTse, Sze-Wah
dc.contributor.authorBroderick, Jennifer A
dc.contributor.authorWei, Mei-Ling
dc.contributor.authorLuo, Min-hua
dc.contributor.authorSmith, Deborah
dc.contributor.authorMcCaffery, Peter J.
dc.contributor.authorStamm, Stefan
dc.contributor.authorAndreadis, Athena
dc.date2022-08-11T08:07:57.000
dc.date.accessioned2022-08-23T15:37:15Z
dc.date.available2022-08-23T15:37:15Z
dc.date.issued2005-09-07
dc.date.submitted2010-09-30
dc.identifier.citationGene. 2005 Oct 10;359:63-72. <a href="http://dx.doi.org/10.1016/j.gene.2005.06.025">Link to article on publisher's site</a>
dc.identifier.issn0378-1119 (Linking)
dc.identifier.doi10.1016/j.gene.2005.06.025
dc.identifier.pmid16143467
dc.identifier.urihttp://hdl.handle.net/20.500.14038/25677
dc.description.abstractIn this study we describe the identification and characterization of a novel cytosolic protein of the guanine exchange factor (GEF) family. The human cDNA corresponds to predicted human protein FLJ00128/FLJ10357 located on chromosome 14q11.2. The deduced protein sequence contains in its C-terminus a RhoGEF domain followed by a pleckstrin domain. Its N-terminus, central region and RhoGEF/pleckstrin domain are homologous to the recently identified zebrafish Quattro protein, which is involved in morphogenetic movements mediated by the actin cytoskeleton. Based on the homology of our protein's RhoGEF domain to the RhoGEF domains of Trio, Duo and Duet and its homology with Quattro, we named it Solo. The Solo mRNA is ubiquitously expressed but enriched in brain, its expression peaks perinatally and it undergoes extensive alternative splicing. In both myoblasts and neuroblastoma cells, the Solo protein is concentrated around the nucleus.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=16143467&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1016/j.gene.2005.06.025
dc.subjectAmino Acid Sequence
dc.subjectAnimals
dc.subjectBlood Proteins
dc.subjectBlotting, Northern
dc.subjectBlotting, Western
dc.subjectBrain
dc.subjectCarrier Proteins
dc.subjectCell Line
dc.subjectCell Line, Tumor
dc.subjectCloning, Molecular
dc.subjectCytoplasm
dc.subjectDNA, Complementary
dc.subjectExons
dc.subject*Gene Expression Profiling
dc.subjectGuanine Nucleotide Exchange Factors
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectImmunoprecipitation
dc.subjectIntrons
dc.subjectMale
dc.subjectMice
dc.subjectMolecular Sequence Data
dc.subjectPhosphoproteins
dc.subjectProtein Binding
dc.subjectRNA, Messenger
dc.subjectRats
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectSaccharomyces cerevisiae
dc.subjectSequence Alignment
dc.subjectSequence Analysis, DNA
dc.subjectSequence Homology, Amino Acid
dc.subjectTwo-Hybrid System Techniques
dc.subjecttau Proteins
dc.subjectCell Biology
dc.titleIdentification, expression analysis, genomic organization and cellular location of a novel protein with a RhoGEF domain
dc.typeJournal Article
dc.source.journaltitleGene
dc.source.volume359
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/andreadis/13
dc.identifier.contextkey1587270
html.description.abstract<p>In this study we describe the identification and characterization of a novel cytosolic protein of the guanine exchange factor (GEF) family. The human cDNA corresponds to predicted human protein FLJ00128/FLJ10357 located on chromosome 14q11.2. The deduced protein sequence contains in its C-terminus a RhoGEF domain followed by a pleckstrin domain. Its N-terminus, central region and RhoGEF/pleckstrin domain are homologous to the recently identified zebrafish Quattro protein, which is involved in morphogenetic movements mediated by the actin cytoskeleton. Based on the homology of our protein's RhoGEF domain to the RhoGEF domains of Trio, Duo and Duet and its homology with Quattro, we named it Solo. The Solo mRNA is ubiquitously expressed but enriched in brain, its expression peaks perinatally and it undergoes extensive alternative splicing. In both myoblasts and neuroblastoma cells, the Solo protein is concentrated around the nucleus.</p>
dc.identifier.submissionpathandreadis/13
dc.contributor.departmentShriver Center
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages63-72


Files in this item

Thumbnail
Name:
Publisher version

This item appears in the following Collection(s)

Show simple item record