CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion
dc.contributor.author | Mou, Haiwei | |
dc.contributor.author | Smith, Jordan L. | |
dc.contributor.author | Peng, Lingtao | |
dc.contributor.author | Moore, Jill | |
dc.contributor.author | Zhang, Xiao-Ou | |
dc.contributor.author | Song, Chun-Qing | |
dc.contributor.author | Sheel, Ankur | |
dc.contributor.author | Ozata, Deniz M | |
dc.contributor.author | Li, Yingxiang | |
dc.contributor.author | Emerson, Charles P. Jr. | |
dc.contributor.author | Sontheimer, Erik J. | |
dc.contributor.author | Moore, Melissa J. | |
dc.contributor.author | Weng, Zhiping | |
dc.contributor.author | Xue, Wen | |
dc.date | 2022-08-11T08:07:58.000 | |
dc.date.accessioned | 2022-08-23T15:37:54Z | |
dc.date.available | 2022-08-23T15:37:54Z | |
dc.date.issued | 2017-06-14 | |
dc.date.submitted | 2017-07-12 | |
dc.identifier.citation | Genome Biol. 2017 Jun 14;18(1):108. doi: 10.1186/s13059-017-1237-8. <a href="https://doi.org/10.1186/s13059-017-1237-8">Link to article on publisher's site</a> | |
dc.identifier.issn | 1474-7596 (Linking) | |
dc.identifier.doi | 10.1186/s13059-017-1237-8 | |
dc.identifier.pmid | 28615073 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/25821 | |
dc.description | <p>Full author list omitted for brevity. For full list of authors see article.</p> | |
dc.description.abstract | CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of beta-catenin exon 3, which encodes an autoinhibitory domain, induces partial skipping of the in-frame exon and nuclear accumulation of beta-catenin. A single sgRNA can induce small insertions or deletions that partially alter splicing or unexpected larger deletions that remove exons. Exon skipping adds to the unexpected outcomes that must be accounted for, and perhaps taken advantage of, in CRISPR experiments. | |
dc.language.iso | en_US | |
dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=28615073&dopt=Abstract">Link to Article in PubMed</a> | |
dc.rights | Copyright © The Author(s). 2017. | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | CRISPR/Cas9 genome editing | |
dc.subject | single-guide RNAs | |
dc.subject | sgRNAs | |
dc.subject | exon skipping | |
dc.subject | Biochemistry, Biophysics, and Structural Biology | |
dc.subject | Bioinformatics | |
dc.subject | Computational Biology | |
dc.subject | Integrative Biology | |
dc.subject | Systems Biology | |
dc.title | CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion | |
dc.type | Journal Article | |
dc.source.journaltitle | Genome biology | |
dc.source.volume | 18 | |
dc.source.issue | 1 | |
dc.identifier.legacyfulltext | https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1118&context=bioinformatics_pubs&unstamped=1 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/bioinformatics_pubs/111 | |
dc.identifier.contextkey | 10417164 | |
refterms.dateFOA | 2022-08-23T15:37:54Z | |
html.description.abstract | <p>CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of beta-catenin exon 3, which encodes an autoinhibitory domain, induces partial skipping of the in-frame exon and nuclear accumulation of beta-catenin. A single sgRNA can induce small insertions or deletions that partially alter splicing or unexpected larger deletions that remove exons. Exon skipping adds to the unexpected outcomes that must be accounted for, and perhaps taken advantage of, in CRISPR experiments.</p> | |
dc.identifier.submissionpath | bioinformatics_pubs/111 | |
dc.contributor.department | Department of Molecular, Cell and Cancer Biology | |
dc.contributor.department | Department of Biochemistry and Molecular Pharmacology | |
dc.contributor.department | Program in Molecular Medicine | |
dc.contributor.department | Wellstone Muscular Dystrophy Program, Department of Neurology | |
dc.contributor.department | Program in Bioinformatics and Integrative Biology | |
dc.contributor.department | RNA Therapeutics Institute | |
dc.contributor.department | Emerson Lab | |
dc.source.pages | 108 |