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dc.contributor.authorMou, Haiwei
dc.contributor.authorSmith, Jordan L.
dc.contributor.authorPeng, Lingtao
dc.contributor.authorMoore, Jill
dc.contributor.authorZhang, Xiao-Ou
dc.contributor.authorSong, Chun-Qing
dc.contributor.authorSheel, Ankur
dc.contributor.authorOzata, Deniz M
dc.contributor.authorLi, Yingxiang
dc.contributor.authorEmerson, Charles P. Jr.
dc.contributor.authorSontheimer, Erik J.
dc.contributor.authorMoore, Melissa J.
dc.contributor.authorWeng, Zhiping
dc.contributor.authorXue, Wen
dc.date2022-08-11T08:07:58.000
dc.date.accessioned2022-08-23T15:37:54Z
dc.date.available2022-08-23T15:37:54Z
dc.date.issued2017-06-14
dc.date.submitted2017-07-12
dc.identifier.citationGenome Biol. 2017 Jun 14;18(1):108. doi: 10.1186/s13059-017-1237-8. <a href="https://doi.org/10.1186/s13059-017-1237-8">Link to article on publisher's site</a>
dc.identifier.issn1474-7596 (Linking)
dc.identifier.doi10.1186/s13059-017-1237-8
dc.identifier.pmid28615073
dc.identifier.urihttp://hdl.handle.net/20.500.14038/25821
dc.description<p>Full author list omitted for brevity. For full list of authors see article.</p>
dc.description.abstractCRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of beta-catenin exon 3, which encodes an autoinhibitory domain, induces partial skipping of the in-frame exon and nuclear accumulation of beta-catenin. A single sgRNA can induce small insertions or deletions that partially alter splicing or unexpected larger deletions that remove exons. Exon skipping adds to the unexpected outcomes that must be accounted for, and perhaps taken advantage of, in CRISPR experiments.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=28615073&dopt=Abstract">Link to Article in PubMed</a>
dc.rightsCopyright © The Author(s). 2017.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCRISPR/Cas9 genome editing
dc.subjectsingle-guide RNAs
dc.subjectsgRNAs
dc.subjectexon skipping
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.subjectBioinformatics
dc.subjectComputational Biology
dc.subjectIntegrative Biology
dc.subjectSystems Biology
dc.titleCRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion
dc.typeJournal Article
dc.source.journaltitleGenome biology
dc.source.volume18
dc.source.issue1
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1118&amp;context=bioinformatics_pubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/bioinformatics_pubs/111
dc.identifier.contextkey10417164
refterms.dateFOA2022-08-23T15:37:54Z
html.description.abstract<p>CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of beta-catenin exon 3, which encodes an autoinhibitory domain, induces partial skipping of the in-frame exon and nuclear accumulation of beta-catenin. A single sgRNA can induce small insertions or deletions that partially alter splicing or unexpected larger deletions that remove exons. Exon skipping adds to the unexpected outcomes that must be accounted for, and perhaps taken advantage of, in CRISPR experiments.</p>
dc.identifier.submissionpathbioinformatics_pubs/111
dc.contributor.departmentDepartment of Molecular, Cell and Cancer Biology
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentWellstone Muscular Dystrophy Program, Department of Neurology
dc.contributor.departmentProgram in Bioinformatics and Integrative Biology
dc.contributor.departmentRNA Therapeutics Institute
dc.contributor.departmentEmerson Lab
dc.source.pages108


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