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dc.contributor.authorDavis, Gregory M.
dc.contributor.authorTu, Shikui
dc.contributor.authorWilce, Jacqueline A.
dc.contributor.authorClaycomb, Julie M.
dc.contributor.authorWeng, Zhiping
dc.contributor.authorBoag, Peter R.
dc.date2022-08-11T08:07:59.000
dc.date.accessioned2022-08-23T15:38:01Z
dc.date.available2022-08-23T15:38:01Z
dc.date.issued2018-12-21
dc.date.submitted2019-01-09
dc.identifier.citation<p>Elife. 2018 Dec 21;7. pii: 35478. doi: 10.7554/eLife.35478. [Epub ahead of print] <a href="https://doi.org/10.7554/eLife.35478">Link to article on publisher's site</a></p>
dc.identifier.issn2050-084X (Linking)
dc.identifier.doi10.7554/eLife.35478
dc.identifier.pmid30575518
dc.identifier.urihttp://hdl.handle.net/20.500.14038/25846
dc.description<p>Full author list omitted for brevity. For the full list of authors, see article.</p>
dc.description.abstractProper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=30575518&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rightsThis is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/
dc.subjectC. elegans
dc.subjectchromosomes
dc.subjectgene expression
dc.subjectgenetics
dc.subjectgenomics
dc.subjectAmino Acids, Peptides, and Proteins
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.subjectBioinformatics
dc.subjectComputational Biology
dc.subjectGenetic Phenomena
dc.subjectGenetics
dc.subjectGenomics
dc.subjectIntegrative Biology
dc.subjectNucleic Acids, Nucleotides, and Nucleosides
dc.titleThe TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans
dc.typeJournal Article
dc.source.journaltitleeLife
dc.source.volume7
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1148&amp;context=bioinformatics_pubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/bioinformatics_pubs/139
dc.identifier.contextkey13591439
refterms.dateFOA2022-08-23T15:38:01Z
html.description.abstract<p>Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.</p>
dc.identifier.submissionpathbioinformatics_pubs/139
dc.contributor.departmentProgram in Bioinformatics and Integrative Biology
dc.source.pagese35478


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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Except where otherwise noted, this item's license is described as This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.