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dc.contributor.authorYu, Tianxiong
dc.contributor.authorKoppetsch, Birgit S.
dc.contributor.authorPagliarani, Sara
dc.contributor.authorJohnston, Stephen
dc.contributor.authorSilverstein, Noah J.
dc.contributor.authorLuban, Jeremy
dc.contributor.authorChappell, Keith
dc.contributor.authorWeng, Zhiping
dc.contributor.authorTheurkauf, William E.
dc.date2022-08-11T08:07:59.000
dc.date.accessioned2022-08-23T15:38:06Z
dc.date.available2022-08-23T15:38:06Z
dc.date.issued2019-10-10
dc.date.submitted2019-11-25
dc.identifier.citation<p>Cell. 2019 Oct 17;179(3):632-643.e12. doi: 10.1016/j.cell.2019.09.002. Epub 2019 Oct 10. <a href="https://doi.org/10.1016/j.cell.2019.09.002">Link to article on publisher's site</a></p>
dc.identifier.issn0092-8674 (Linking)
dc.identifier.doi10.1016/j.cell.2019.09.002
dc.identifier.pmid31607510
dc.identifier.urihttp://hdl.handle.net/20.500.14038/25865
dc.description.abstractAntisense Piwi-interacting RNAs (piRNAs) guide silencing of established transposons during germline development, and sense piRNAs drive ping-pong amplification of the antisense pool, but how the germline responds to genome invasion is not understood. The KoRV-A gammaretrovirus infects the soma and germline and is sweeping through wild koalas by a combination of horizontal and vertical transfer, allowing direct analysis of retroviral invasion of the germline genome. Gammaretroviruses produce spliced Env mRNAs and unspliced transcripts encoding Gag, Pol, and the viral genome, but KoRV-A piRNAs are almost exclusively derived from unspliced genomic transcripts and are strongly sense-strand biased. Significantly, selective piRNA processing of unspliced proviral transcripts is conserved from insects to placental mammals. We speculate that bypassed splicing generates a conserved molecular pattern that directs proviral genomic transcripts to the piRNA biogenesis machinery and that this "innate" piRNA response suppresses transposition until antisense piRNAs are produced, establishing sequence-specific adaptive immunity.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=31607510&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1016/j.cell.2019.09.002
dc.subjectAKV
dc.subjectKoRV-A
dc.subjectgenome invasion
dc.subjectgerm-line genome
dc.subjectkoala
dc.subjectpiRNA
dc.subjectpiRNA clusters
dc.subjectretrovirus
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.subjectComputational Biology
dc.subjectGenetic Phenomena
dc.subjectGenetics and Genomics
dc.subjectHemic and Immune Systems
dc.subjectNucleic Acids, Nucleotides, and Nucleosides
dc.titleThe piRNA Response to Retroviral Invasion of the Koala Genome
dc.typeJournal Article
dc.source.journaltitleCell
dc.source.volume179
dc.source.issue3
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/bioinformatics_pubs/158
dc.identifier.contextkey15863121
html.description.abstract<p>Antisense Piwi-interacting RNAs (piRNAs) guide silencing of established transposons during germline development, and sense piRNAs drive ping-pong amplification of the antisense pool, but how the germline responds to genome invasion is not understood. The KoRV-A gammaretrovirus infects the soma and germline and is sweeping through wild koalas by a combination of horizontal and vertical transfer, allowing direct analysis of retroviral invasion of the germline genome. Gammaretroviruses produce spliced Env mRNAs and unspliced transcripts encoding Gag, Pol, and the viral genome, but KoRV-A piRNAs are almost exclusively derived from unspliced genomic transcripts and are strongly sense-strand biased. Significantly, selective piRNA processing of unspliced proviral transcripts is conserved from insects to placental mammals. We speculate that bypassed splicing generates a conserved molecular pattern that directs proviral genomic transcripts to the piRNA biogenesis machinery and that this "innate" piRNA response suppresses transposition until antisense piRNAs are produced, establishing sequence-specific adaptive immunity.</p>
dc.identifier.submissionpathbioinformatics_pubs/158
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentProgram in Bioinformatics and Integrative Biology
dc.source.pages632-643.e12


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