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    Ribosomes guide pachytene piRNA formation on long intergenic piRNA precursors

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    Authors
    Sun, Yu H.
    Zhu, Jiang
    Xie, Li Huitong
    Li, Ziwei
    Meduri, Rajyalakshmi
    Zhu, Xiaopeng
    Song, Chi
    Chen, Chen
    Ricci, Emiliano P.
    Weng, Zhiping
    Li, Xin Zhiguo
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    UMass Chan Affiliations
    Program in Bioinformatics and Integrative Biology
    Document Type
    Journal Article
    Publication Date
    2020-02-03
    Keywords
    RNA
    Spermatogenesis
    Biochemistry, Biophysics, and Structural Biology
    Bioinformatics
    Cell Biology
    Computational Biology
    Developmental Biology
    Nucleic Acids, Nucleotides, and Nucleosides
    Reproductive and Urinary Physiology
    
    Metadata
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    Link to Full Text
    https://doi.org/10.1038/s41556-019-0457-4
    Abstract
    PIWI-interacting RNAs (piRNAs) are a class of small non-coding RNAs essential for fertility. In adult mouse testes, most piRNAs are derived from long single-stranded RNAs lacking annotated open reading frames (ORFs). The mechanisms underlying how piRNA sequences are defined during the cleavages of piRNA precursors remain elusive. Here, we show that 80S ribosomes translate the 5'-proximal short ORFs (uORFs) of piRNA precursors. The MOV10L1/Armitage RNA helicase then facilitates the translocation of ribosomes into the uORF downstream regions (UDRs). The ribosome-bound UDRs are targeted by piRNA processing machinery, with the processed ribosome-protected regions becoming piRNAs. The dual modes of interaction between ribosomes and piRNA precursors underlie the distinct piRNA biogenesis requirements at uORFs and UDRs. Ribosomes also mediate piRNA processing in roosters and green lizards, implying that this mechanism is evolutionarily conserved in amniotes. Our results uncover a function for ribosomes on non-coding regions of RNAs and reveal the mechanisms underlying how piRNAs are defined.
    Source

    Sun YH, Zhu J, Xie LH, Li Z, Meduri R, Zhu X, Song C, Chen C, Ricci EP, Weng Z, Li XZ. Ribosomes guide pachytene piRNA formation on long intergenic piRNA precursors. Nat Cell Biol. 2020 Feb;22(2):200-212. doi: 10.1038/s41556-019-0457-4. Epub 2020 Feb 3. Erratum in: Nat Cell Biol. 2020 Mar;22(3):353. PMID: 32015435. Link to article on publisher's site

    DOI
    10.1038/s41556-019-0457-4
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/25873
    PubMed ID
    32015435
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    Link to Article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.1038/s41556-019-0457-4
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