Target RNA-directed tailing and trimming purifies the sorting of endo-siRNAs between the two Drosophila Argonaute proteins
UMass Chan Affiliations
Program in Bioinformatics and Integrative BiologyDepartment of Biochemistry and Molecular Pharmacology
Document Type
Journal ArticlePublication Date
2011-01-01Keywords
AnimalsArgonaute Proteins
Base Pairing
Base Sequence
Cross-Linking Reagents
Drosophila Proteins
Drosophila melanogaster
Eukaryotic Initiation Factors
Immunoprecipitation
MicroRNAs
Molecular Sequence Data
Protein Binding
RNA
RNA, Messenger
RNA, Small Interfering
RNA-Induced Silencing Complex
Bioinformatics
Computational Biology
Genetics and Genomics
Molecular Biology
Systems Biology
Metadata
Show full item recordAbstract
In flies, 22-23-nucleotide (nt) microRNA duplexes typically contain mismatches and begin with uridine, so they bind Argonaute1 (Ago1), whereas 21-nt siRNA duplexes are perfectly paired and begin with cytidine, promoting their loading into Ago2. A subset of Drosophila endogenous siRNAs-the hairpin-derived hp-esiRNAs-are born as mismatched duplexes that often begin with uridine. These would be predicted to load into Ago1, yet accumulate at steady-state bound to Ago2. In vitro, such hp-esiRNA duplexes assemble into Ago1. In vivo, they encounter complementary target mRNAs that trigger their tailing and trimming, causing Ago1-loaded hp-esiRNAs to be degraded. In contrast, Ago2-associated hp-esiRNAs are 2'-O-methyl modified at their 3' ends, protecting them from tailing and trimming. Consequently, the steady-state distribution of esiRNAs reflects not only their initial sorting between Ago1 and Ago2 according to their duplex structure, length, and first nucleotide, but also the targeted destruction of the single-stranded small RNAs after their loading into an Argonaute protein.Source
RNA. 2011 Jan;17(1):54-63. doi: 10.1261/rna.2498411. Link to article on publisher's siteDOI
10.1261/rna.2498411Permanent Link to this Item
http://hdl.handle.net/20.500.14038/25901PubMed ID
21106652Related Resources
Link to article in PubMedRights
Freely available online through the RNA Open Access option. This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported License), as described at http://creativecommons.org/licenses/by-nc/3.0/.ae974a485f413a2113503eed53cd6c53
10.1261/rna.2498411