Pitfalls of mapping high-throughput sequencing data to repetitive sequences: Piwi's genomic targets still not identified
AuthorsMarinov, Georgi K.
Wold, Barbara J.
Hannon, Gregory J.
Aravin, Alexei A.
Zamore, Phillip D.
Toth, Katalin Fejes
UMass Chan AffiliationsDepartment of Biochemistry and Molecular Pharmacology
Program in Bioinformatics and Integrative Biology
High-Throughput Nucleotide Sequencing
RNA Polymerase II
RNA, Small Interfering
Sequence Analysis, DNA
Biochemistry, Biophysics, and Structural Biology
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AbstractHuang et al. (2013) recently reported that chromatin immunoprecipitation sequencing (ChIP-seq) reveals the genome-wide sites of occupancy by Piwi, a piRNA-guided Argonaute protein central to transposon silencing in Drosophila. Their study also reported that loss of Piwi causes widespread rewiring of transcriptional patterns, as evidenced by changes in RNA polymerase II occupancy across the genome. Here we reanalyze their data and report that the underlying deep-sequencing dataset does not support the authors' genome-wide conclusions.
SourceDev Cell. 2015 Mar 23;32(6):765-71. doi: 10.1016/j.devcel.2015.01.013. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/25904
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