Pitfalls of mapping high-throughput sequencing data to repetitive sequences: Piwi's genomic targets still not identified
Name:
Publisher version
UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyProgram in Bioinformatics and Integrative Biology
Document Type
Journal ArticlePublication Date
2015-03-23Keywords
AnimalsArgonaute Proteins
Base Sequence
Binding Sites
Chromatin Immunoprecipitation
DNA-Binding Proteins
Drosophila Proteins
Drosophila melanogaster
Genome
High-Throughput Nucleotide SequencingMethyltransferasesRNA InterferenceRNA Polymerase IIRNA, Small InterferingSequence Analysis, DNABiochemistry, Biophysics, and Structural BiologyBioinformaticsComputational BiologyIntegrative BiologySystems Biology
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Huang et al. (2013) recently reported that chromatin immunoprecipitation sequencing (ChIP-seq) reveals the genome-wide sites of occupancy by Piwi, a piRNA-guided Argonaute protein central to transposon silencing in Drosophila. Their study also reported that loss of Piwi causes widespread rewiring of transcriptional patterns, as evidenced by changes in RNA polymerase II occupancy across the genome. Here we reanalyze their data and report that the underlying deep-sequencing dataset does not support the authors' genome-wide conclusions.Source
Dev Cell. 2015 Mar 23;32(6):765-71. doi: 10.1016/j.devcel.2015.01.013. Link to article on publisher's siteDOI
10.1016/j.devcel.2015.01.013Permanent Link to this Item
http://hdl.handle.net/20.500.14038/25904PubMed ID
25805138Related Resources
Link to Article in PubMedScopus Count
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