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    The initial uridine of primary piRNAs does not create the tenth adenine that Is the hallmark of secondary piRNAs

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    Authors
    Wang, Wei
    Yoshikawa, Mayu
    Han, Bo W.
    Izumi, Natsuko
    Tomari, Yukihide
    Weng, Zhiping
    Zamore, Phillip D.
    UMass Chan Affiliations
    Program in Bioinformatics and Integrative Biology
    Department of Biochemistry and Molecular Pharmacology
    RNA Therapeutics Institute
    Document Type
    Journal Article
    Publication Date
    2014-12-04
    Keywords
    Adenine
    Animals
    Argonaute Proteins
    Bombyx
    Drosophila Proteins
    Drosophila melanogaster
    Mice
    Models, Genetic
    Molecular Sequence Data
    Peptide Initiation Factors
    RNA, Small Interfering
    Uridine
    Biochemistry, Biophysics, and Structural Biology
    Bioinformatics
    Computational Biology
    Integrative Biology
    Systems Biology
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    Link to Full Text
    http://dx.doi.org/10.1016/j.molcel.2014.10.016
    Abstract
    PIWI-interacting RNAs (piRNAs) silence transposons in animal germ cells. PIWI proteins bind and amplify piRNAs via the "Ping-Pong" pathway. Because PIWI proteins cleave RNAs between target nucleotides t10 and t11-the nucleotides paired to piRNA guide positions g10 and g11-the first ten nucleotides of piRNAs participating in the Ping-Pong amplification cycle are complementary. Drosophila piRNAs bound to the PIWI protein Aubergine typically begin with uridine (1U), while piRNAs bound to Argonaute3, which are produced by Ping-Pong amplification, often have adenine at position 10 (10A). The Ping-Pong model proposes that the 10A is a consequence of 1U. We find that 10A is not caused by 1U. Instead, fly Aubergine as well as its homologs, Siwi in silkmoth and MILI in mice, have an intrinsic preference for adenine at the t1 position of their target RNAs; during Ping-Pong amplification, this t1A subsequently becomes the g10A of a piRNA bound to Argonaute3.
    Source
    Wang W, Yoshikawa M, Han BW, Izumi N, Tomari Y, Weng Z, Zamore PD. The initial uridine of primary piRNAs does not create the tenth adenine that Is the hallmark of secondary piRNAs. Mol Cell. 2014 Dec 4;56(5):708-16. doi: 10.1016/j.molcel.2014.10.016. Epub 2014 Nov 20. PubMed PMID: 25453759; PubMed Central PMCID: PMC4337030. Link to article on publisher's website
    DOI
    10.1016/j.molcel.2014.10.016
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/25926
    PubMed ID
    25453759
    Related Resources
    Link to article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.molcel.2014.10.016
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