Slicing and Binding by Ago3 or Aub Trigger Piwi-Bound piRNA Production by Distinct Mechanisms
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Authors
Wang, WeiHan, Bo W.
Tipping, Cindy
Ge, Daniel Tianfang
Zhang, Zhao
Weng, Zhiping
Zamore, Phillip D.
UMass Chan Affiliations
RNA Therapeutics InstituteDepartment of Biochemistry and Molecular Pharmacology
Program in Bioinformatics and Integrative Biology
Document Type
Journal ArticlePublication Date
2015-09-03Keywords
Active Transport, Cell NucleusAnimals
Argonaute Proteins
DNA Transposable Elements
Drosophila Proteins
Drosophila melanogaster
Female
Gene Silencing
Genes, Insect
Models, Biological
Mutation
Ovum
Peptide Initiation Factors
Protein Binding
RNA Cleavage
RNA, Small Interfering
Biochemistry
Bioinformatics
Computational Biology
Molecular Biology
Molecular Genetics
Metadata
Show full item recordAbstract
In Drosophila ovarian germ cells, PIWI-interacting RNAs (piRNAs) direct Aubergine and Argonaute3 to cleave transposon transcripts and instruct Piwi to repress transposon transcription, thereby safeguarding the germline genome. Here, we report that RNA cleavage by Argonaute3 initiates production of most Piwi-bound piRNAs. We find that the cardinal function of Argonaute3, whose piRNA guides predominantly correspond to sense transposon sequences, is to produce antisense piRNAs that direct transcriptional silencing by Piwi, rather than to make piRNAs that guide post-transcriptional silencing by Aubergine. We also find that the Tudor domain protein Qin prevents Aubergine's cleavage products from becoming Piwi-bound piRNAs, ensuring that antisense piRNAs guide Piwi. Although Argonaute3 slicing is required to efficiently trigger phased piRNA production, an alternative, slicing-independent pathway suffices to generate Piwi-bound piRNAs that repress transcription of a subset of transposon families. This alternative pathway may help flies silence newly acquired transposons for which they lack extensively complementary piRNAs.Source
Mol Cell. 2015 Sep 3;59(5):819-30. doi: 10.1016/j.molcel.2015.08.007. Link to article on publisher's siteDOI
10.1016/j.molcel.2015.08.007Permanent Link to this Item
http://hdl.handle.net/20.500.14038/25933PubMed ID
26340424Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/j.molcel.2015.08.007