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dc.contributor.authorVan Treuren, Will
dc.contributor.authorPonnusamy, Loganathan
dc.contributor.authorBrinkerhoff, R Jory
dc.contributor.authorGonzalez, Antonio
dc.contributor.authorParobek, Christian M.
dc.contributor.authorJuliano, Jonathan J.
dc.contributor.authorAndreadis, Theodore G.
dc.contributor.authorFalco, Richard C.
dc.contributor.authorZiegler, Lorenza Beati
dc.contributor.authorHathaway, Nicholas J
dc.contributor.authorKeeler, Corinna
dc.contributor.authorEmch, Michael
dc.contributor.authorBailey, Jeffrey A.
dc.contributor.authorRoe, R. Michael
dc.contributor.authorApperson, Charles S.
dc.contributor.authorKnight, Rob
dc.contributor.authorMeshnick, Steven R.
dc.date2022-08-11T08:07:59.000
dc.date.accessioned2022-08-23T15:38:26Z
dc.date.available2022-08-23T15:38:26Z
dc.date.issued2015-09-01
dc.date.submitted2016-01-07
dc.identifier.citationAppl Environ Microbiol. 2015 Sep;81(18):6200-9. doi: 10.1128/AEM.01562-15. Epub 2015 Jul 6. <a href="http://dx.doi.org/10.1128/AEM.01562-15">Link to article on publisher's site</a>
dc.identifier.issn0099-2240 (Linking)
dc.identifier.doi10.1128/AEM.01562-15
dc.identifier.pmid26150449
dc.identifier.urihttp://hdl.handle.net/20.500.14038/25934
dc.description.abstractIxodes scapularis is the principal vector of Lyme disease on the East Coast and in the upper Midwest regions of the United States, yet the tick is also present in the Southeast, where Lyme disease is absent or rare. A closely related species, I. affinis, also carries the pathogen in the South but does not seem to transmit it to humans. In order to better understand the geographic diversity of the tick, we analyzed the microbiota of 104 adult I. scapularis and 13 adult I. affinis ticks captured in 19 locations in South Carolina, North Carolina, Virginia, Connecticut, and New York. Initially, ticks from 4 sites were analyzed by 454 pyrosequencing. Subsequently, ticks from these sites plus 15 others were analyzed by sequencing with an Illumina MiSeq machine. By both analyses, the microbiomes of female ticks were significantly less diverse than those of male ticks. The dissimilarity between tick microbiomes increased with distance between sites, and the state in which a tick was collected could be inferred from its microbiota. The genus Rickettsia was prominent in all locations. Borrelia was also present in most locations and was present at especially high levels in one site in western Virginia. In contrast, members of the family Enterobacteriaceae were very common in North Carolina I. scapularis ticks but uncommon in I. scapularis ticks from other sites and in North Carolina I. affinis ticks. These data suggest substantial variations in the Ixodes microbiota in association with geography, species, and sex.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=26150449&dopt=Abstract">Link to Article in PubMed</a>
dc.rightsPublisher PDF posted as allowed by the publisher's author rights policy at http://journals.asm.org/site/misc/ASM_Author_Statement.xhtml.
dc.subjectBioinformatics
dc.subjectComputational Biology
dc.subjectEnvironmental Microbiology and Microbial Ecology
dc.titleVariation in the Microbiota of Ixodes Ticks with Regard to Geography, Species, and Sex
dc.typeJournal Article
dc.source.journaltitleApplied and environmental microbiology
dc.source.volume81
dc.source.issue18
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1082&amp;context=bioinformatics_pubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/bioinformatics_pubs/75
dc.legacy.embargo2016-03-01T00:00:00-08:00
dc.identifier.contextkey7992043
refterms.dateFOA2022-08-23T15:38:26Z
html.description.abstract<p>Ixodes scapularis is the principal vector of Lyme disease on the East Coast and in the upper Midwest regions of the United States, yet the tick is also present in the Southeast, where Lyme disease is absent or rare. A closely related species, I. affinis, also carries the pathogen in the South but does not seem to transmit it to humans. In order to better understand the geographic diversity of the tick, we analyzed the microbiota of 104 adult I. scapularis and 13 adult I. affinis ticks captured in 19 locations in South Carolina, North Carolina, Virginia, Connecticut, and New York. Initially, ticks from 4 sites were analyzed by 454 pyrosequencing. Subsequently, ticks from these sites plus 15 others were analyzed by sequencing with an Illumina MiSeq machine. By both analyses, the microbiomes of female ticks were significantly less diverse than those of male ticks. The dissimilarity between tick microbiomes increased with distance between sites, and the state in which a tick was collected could be inferred from its microbiota. The genus Rickettsia was prominent in all locations. Borrelia was also present in most locations and was present at especially high levels in one site in western Virginia. In contrast, members of the family Enterobacteriaceae were very common in North Carolina I. scapularis ticks but uncommon in I. scapularis ticks from other sites and in North Carolina I. affinis ticks. These data suggest substantial variations in the Ixodes microbiota in association with geography, species, and sex.</p>
dc.identifier.submissionpathbioinformatics_pubs/75
dc.contributor.departmentProgram in Bioinformatics and Integrative Biology
dc.source.pages6200-9


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